The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
Use at an assay dependent concentration. PubMed: 24936062
Application notesIs unsuitable for IP.
FunctionComponent of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.
Tissue specificityHighly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the FBXO31 family. Contains 1 F-box domain.
Developmental stageExpression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level).
Post-translational modificationsPhosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization.
All lanes : Anti-FBXO31 antibody (ab86137) at 0.4 µg/ml
Lane 1 : Whole cell lysate from 293T cells Lane 2 : Whole cell lysate from mouse NIH3T3 cells
Lysates/proteins at 50 µg per lane.
Predicted band size : 61 kDa Observed band size : 61 kDa
Exposure time : 30 seconds
References for Anti-FBXO31 antibody (ab86137)
This product has been referenced in:
Liu J et al. F-box only protein 31 (FBXO31) negatively regulates p38 mitogen-activated protein kinase (MAPK) signaling by mediating lysine 48-linked ubiquitination and degradation of mitogen-activated protein kinase kinase 6 (MKK6). J Biol Chem289:21508-18 (2014).
Read more (PubMed: 24936062) »