Overview

  • Product name
  • Description
    Rabbit polyclonal to FBXO31
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within the amino acids 489-539 of Human FBXO31, NP_079011.3

  • Positive control
    • Whole cell lysate from 293T and mouse NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab86137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 24936062
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor.
    • Tissue specificity
      Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the FBXO31 family.
      Contains 1 F-box domain.
    • Developmental stage
      Expression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level).
    • Post-translational
      modifications
      Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp434B027 antibody
      • DKFZp434J1815 antibody
      • F box only protein 31 antibody
      • F box protein 31 antibody
      • F-box only protein 31 antibody
      • FBX14 antibody
      • FBX31 antibody
      • FBX31_HUMAN antibody
      • FBXO14 antibody
      • Fbxo31 antibody
      • FLJ22477 antibody
      • MGC15419 antibody
      • MGC9527 antibody
      • pp2386 antibody
      • Putative breast cancer tumor suppressor antibody
      • SCF ubiquitin ligase specificity factor antibody
      see all

    Images

    • All lanes : Anti-FBXO31 antibody (ab86137) at 0.4 µg/ml

      Lane 1 : Whole cell lysate from 293T cells
      Lane 2 : Whole cell lysate from mouse NIH3T3 cells

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 61 kDa
      Observed band size : 61 kDa


      Exposure time : 30 seconds

    References

    This product has been referenced in:
    • Liu J  et al. F-box only protein 31 (FBXO31) negatively regulates p38 mitogen-activated protein kinase (MAPK) signaling by mediating lysine 48-linked ubiquitination and degradation of mitogen-activated protein kinase kinase 6 (MKK6). J Biol Chem 289:21508-18 (2014). ICC/IF ; Human . Read more (PubMed: 24936062) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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