Overview

  • Product nameAnti-FCER1G antibodySee all FCER1G primary antibodies ...
  • Description
    Rabbit polyclonal to FCER1G
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MIPAVVLLLL LLVEQAAALG EPQLCYILDA ILFLYGIVLT LLYCRLKIQV) of Human FCER1G (NP_004097).

  • Positive control
    • HeLa cell lysate IHC-P (FFPE): Human Lung (Normal)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab108228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionAssociates with a variety of FcR alpha chains to form a functional signaling complex. Regulates several aspects of the immune response. The gamma subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface.
  • Sequence similaritiesBelongs to the CD3Z/FCER1G family.
    Contains 1 ITAM domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide antibody
    • Fc receptor gamma chain antibody
    • Fc-epsilon RI-gamma antibody
    • FCER1G antibody
    • FCERG_HUMAN antibody
    • FceRI gamma antibody
    • FCRG antibody
    • FcRgamma antibody
    • High affinity immunoglobulin epsilon receptor subunit gamma antibody
    • IgE Fc receptor subunit gamma antibody
    • Immunoglobulin E receptor, high affinity, gamma chain antibody
    see all

Anti-FCER1G antibody images

  • IHC image of FCER1G staining in Human Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108228, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Anti-FCER1G antibody (ab108228) at 1 µg/ml + HeLa cell lysate at 10 µg

    Predicted band size : 10 kDa
    10-20% gel

References for Anti-FCER1G antibody (ab108228)

ab108228 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"