Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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ab462 has been referenced in 6 publications.
Publishing research using ab462? Please let us know so that we can cite the reference in this datasheet
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ab462 detecting FEN1 (green) in Hela Cells by ICC/IF. Cells were counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details.
Image courtesy of Dr. Kirk McManus
ab462 (2µg/ml) staining FEN1 in human duodenum sing an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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