You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-FEN1 antibody [4E7] (ab462)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-FEN1 antibody [4E7]
See all FEN1 products (14) ...

Description

Mouse monoclonal [4E7] to FEN1

Tested applications

Flow Cyt, WB, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant human FEN-1 protein encoding amino acids 1-380 purified from baculovirus-infected Sf-9 cells.

Positive control

MCF-7 cells, recombinant fusion protein.

General notes

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

4E7

Myeloma

NS1

Isotype

IgG1

Light chain type

kappa

Research areas

Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair

  • Immunocytochemistry/ Immunofluorescence - FEN1 antibody [4E7] (ab462)Immunocytochemistry/ Immunofluorescence - FEN1 antibody [4E7] (ab462) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-FEN1 antibody [4E7](ab462)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-FEN1 antibody [4E7](ab462) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Sequence similarities

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.

Post-translational
modifications

Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.

Cellular localization

Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.

Target information above from: UniProt accessionP39748 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DNase IV antibody
  • FEN-1 antibody
  • FEN1 antibody
  • FEN1_HUMAN antibody
  • Flap endonuclease 1 antibody
  • Flap structure specific endonuclease 1 antibody
  • Flap structure-specific endonuclease 1 antibody
  • hFEN-1 antibody
  • hFEN1 antibody
  • Maturation factor 1 antibody
  • MF1 antibody
  • Rad2 antibody
see all

Database links

Anti-FEN1 antibody [4E7] images:

  Immunocytochemistry/ Immunofluorescence - FEN1 antibody [4E7] (ab462)

Immunocytochemistry/ Immunofluorescence - FEN1 antibody [4E7] (ab462)

ab462 detecting FEN1 (green) in Hela Cells by ICC/IF. Cells were counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details.

Image courtesy of Dr. Kirk McManus

See Abreview

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-FEN1 antibody [4E7](ab462)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-FEN1 antibody [4E7](ab462)

ab462 (2µg/ml) staining FEN1 in human duodenum sing an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-FEN1 antibody [4E7] (ab462)

This product has been referenced in:

  • Ruhanen Het al. Involvement of DNA ligase III and ribonuclease H1 in mitochondrial DNA replication in cultured human cells. Biochim Biophys Acta 1813:2000-2007 (2011). WB; Human.Read more (PubMed: 21878356) »
  • Speina Eet al. Human RECQL5{beta} stimulates flap endonuclease 1. Nucleic Acids Res : (2010). ICC/IF; Human.Read more (PubMed: 20081208) »

See all 6 publications for this product

Publishing research using ab462? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"