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Immunocytochemistry/ Immunofluorescence - FEN1 antibody [4E7] (ab462)
![Immunocytochemistry/ Immunofluorescence - FEN1 antibody [4E7] (ab462)](/ps/datasheet/images/ab462_2.jpg)
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Product Name
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FEN1 antibody [4E7]
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See all FEN1 antibodies (12)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [4E7] to FEN1
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Immunogen
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Recombinant human FEN-1 protein encoding amino acids 1-380 purified from baculovirus-infected Sf-9 cells.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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Flow Cyt, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Used at a dilution of 1/300 for 15 hrs on breast cancer cell lines (see Abreview submitted on the 16th August, 2006).
ICC/IF: 1/500 (please see abreview).
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 43 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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MCF-7 cells, recombinant fusion protein.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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Relevance
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FEN 1 (Flap Endonuclease and Five prime Exonuclease 1) functions as an endonuclease when it cleaves branched DNA structures containing single stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN 1 endonuclease and exonuclease activities are stimulated by an association with Proliferating Cell Nuclear Antigen (PCNA) in the nucleus. FEN 1 is believed to have important roles in DNA replication and repair and is required in cell free DNA replication systems.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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4E7
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Myeloma
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NS1
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.4
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FEN1 antibody [4E7] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FEN1 antibody [4E7] images, references, products related to ab462 and other tools.
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FEN1 antibody [4E7] images:
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 Immunocytochemistry/ Immunofluorescence - FEN1 antibody [4E7] (ab462)
ab462 detecting FEN1 (green) in Hela Cells by ICC/IF. Cells were counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details. Image courtesy of Dr. Kirk McManus
See Abreview
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References for FEN1 antibody [4E7] (ab462)
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This product has been used in: (two most recent references)
Schurman SH et al. Direct and indirect roles of RECQL4 in modulating base excision repair capacity. Hum Mol Genet 18:3470-83 (2009). PubMed: 19567405
Akbari M et al. Extracts of proliferating and non-proliferating human cells display different base excision pathways and repair fidelity. DNA Repair (Amst) 8:834-43 (2009). WB; Human. PubMed: 19442590
See all 4 publication references for this product.
If you publish research using ab462 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to FEN1
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FEN1 antibody [4E7] - more information
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Customer reviews (feedback) regarding FEN1 antibody [4E7] |
Customer FAQs regarding FEN1 antibody [4E7] |
Protocols for FEN1 antibody [4E7] |
Price and availability of products related to FEN1 antibody [4E7] |
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FEN1 antibody [4E7] - related products:
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FEN1 antibody [4E7] - other tools:
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Contact Abcam with a Technical Enquiry about FEN1 antibody [4E7] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - FEN1 antibody [4E7] (ab462)](/index.html?datasheet=462#ab462_2.jpg)
