Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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I would like to test ab17993 for use in IHC |
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ANSWER: |
DISCOUNT CODE: *** Expiration date: April 28th, 2012 I am very pleased to hear you would like to accept our offer and test ab17993 in IHC. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 42 kDa
Western blot using ab17993 on HeLa whole cell lysate.
Lane 1: ab17993 at 1µg/ml
Lane 2: ab17993 at 0.1µg/ml
Lane 3: ab17993 at 0.05µg/ml
Lane 4: ab17993 at 0.03µg/ml
Lysates: 20 µg/lane.
Secondary antibody: goat anti-Rabbit HRP conjugate at 1:40,000 (for 0.05 and 0.03 µg/ml anti-Fen1) or 1:60,000 (for 1 and 0.1 µg/ml anti-Fen1). Detection: Chemiluminescence with 15 sec exposure.
ab17993 (1/1000) staining FEN1 in HeLa cells. Secondary antibody: goat anti-rabbit IgG conjugated to Cy3 ®. Cells were counterstained with DAPI. Please refer to Abreview for further details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
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