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- FEN1 antibody (ab17993)
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Immunocytochemistry/ Immunofluorescence - FEN1 antibody (ab17993)
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Product Name
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FEN1 antibody
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See all FEN1 antibodies (12)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FEN1
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Immunogen
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The peptide is derived from a portion of the latter half of the N-terminal domain as defined in Swiss-Prot entry P39748 (this portion is within exon 2).
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Reacts with
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Hu
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Predicted to react
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Ms
(Due to sequence homology)
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Tested applications
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ICC/IF, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: Use at 1:1000 (See Abreview Feb 20 2007)
IP: Use at 4µg/mg of lysate.
WB: Use at a concentration of 0.1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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Relevance
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FEN 1 (Flap Endonuclease and Five prime Exonuclease 1) functions as an endonuclease when it cleaves branched DNA structures containing single stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN 1 endonuclease and exonuclease activities are stimulated by an association with Proliferating Cell Nuclear Antigen (PCNA) in the nucleus. FEN 1 is believed to have important roles in DNA replication and repair and is required in cell free DNA replication systems.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FEN1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FEN1 antibody images, references, products related to ab17993 and other tools.
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FEN1 antibody images:
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 - FEN1 antibody (ab17993)
Predicted band size : 42 kDa
Western blot using ab17993 on HeLa whole cell lysate.
Lane 1: ab17993 at 1µg/ml
Lane 2: ab17993 at 0.1µg/ml
Lane 3: ab17993 at 0.05µg/ml
Lane 4: ab17993 at 0.03µg/ml
Lysates: 20 µg/lane.
Secondary antibody: goat anti-Rabbit HRP conjugate at 1:40,000 (for 0.05 and 0.03 µg/ml anti-Fen1) or 1:60,000 (for 1 and 0.1 µg/ml anti-Fen1). Detection: Chemiluminescence with 15 sec exposure.
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Immunocytochemistry/ Immunofluorescence - FEN1 antibody (ab17993)
ab17993 (1/1000) staining FEN1 in HeLa cells. Secondary antibody: goat anti-rabbit IgG conjugated to Cy3 ®. Cells were counterstained with DAPI. Please refer to Abreview for further details. This image is courtesy of an Abreview submitted by Dr Kirk McManus
See Abreview
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References for FEN1 antibody (ab17993)
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This product has been used in: (two most recent references)
Balakrishnan L et al. Acetylation of Dna2 endonuclease/helicase and flap endonuclease 1 by p300 promotes DNA stability by creating long flap intermediates. J Biol Chem 285:4398-404 (2010). WB; Human. PubMed: 20019387
Balakrishnan L et al. Long patch base excision repair proceeds via coordinated stimulation of the multienzyme DNA repair complex. J Biol Chem 284:15158-72 (2009). WB; Human. PubMed: 19329425
If you publish research using ab17993 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to FEN1
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FEN1 antibody - more information
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Customer reviews (feedback) regarding FEN1 antibody |
Customer FAQs regarding FEN1 antibody |
Protocols for FEN1 antibody |
Price and availability of products related to FEN1 antibody |
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FEN1 antibody - related products:
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FEN1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about FEN1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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