Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Overview
Product name
Anti-FEN1 antibody
See all FEN1 products (14) ...
Description
Rabbit polyclonal to FEN1
Tested applications
ICC/IF, WB, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
The peptide is derived from a portion of the latter half of the N-terminal domain as defined in Swiss-Prot entry P39748 (this portion is within exon 2).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Applications
Show applications key
Our Abpromise guarantee covers the use of ab17993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/1000(See Abreview Feb 20 2007.)
WB: Use a concentration of 0.1 µg/mlDetects a band of approximately 47 kDa (predicted molecular weight: 42 kDa).
IP: Use at 4 µg/mg of lysate.
Target
Function
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Sequence similarities
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
Post-translational
modifications
Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
Cellular localization
Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
Target information above from: UniProt accessionP39748
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNase IV antibody
- FEN-1 antibody
- FEN1 antibody
see all
Database links
- Entrez Gene: 2237 Human
- Entrez Gene: 14156 Mouse
- Omim: 600393 Human
- SwissProt: P39748 Human
- SwissProt: P39749 Mouse
- Unigene: 409065 Human
- Unigene: 2952 Mouse
- Unigene: 470595 Mouse
Anti-FEN1 antibody images:
Western blot - Anti-FEN1 antibody (ab17993)
Predicted band size : 42 kDa
Western blot using ab17993 on HeLa whole cell lysate.
Lane 1: ab17993 at 1µg/ml
Lane 2: ab17993 at 0.1µg/ml
Lane 3: ab17993 at 0.05µg/ml
Lane 4: ab17993 at 0.03µg/ml
Lysates: 20 µg/lane.
Secondary antibody: goat anti-Rabbit HRP conjugate at 1:40,000 (for 0.05 and 0.03 µg/ml anti-Fen1) or 1:60,000 (for 1 and 0.1 µg/ml anti-Fen1). Detection: Chemiluminescence with 15 sec exposure.
Immunocytochemistry/ Immunofluorescence - FEN1 antibody (ab17993)
ab17993 (1/1000) staining FEN1 in HeLa cells. Secondary antibody: goat anti-rabbit IgG conjugated to Cy3 ®. Cells were counterstained with DAPI. Please refer to Abreview for further details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
References for Anti-FEN1 antibody (ab17993)
This product has been referenced in:
- Balakrishnan Let al. Acetylation of Dna2 endonuclease/helicase and flap endonuclease 1 by p300 promotes DNA stability by creating long flap intermediates. J Biol Chem 285:4398-404 (2010). WB; Human.Read more (PubMed: 20019387) »
- Henry RAet al. Components of the secondary pathway stimulate the primary pathway of eukaryotic Okazaki fragment processing. J Biol Chem 285:28496-505 (2010). WB.Read more (PubMed: 20628185) »
See all 3 publications for this product
Publishing research using ab17993? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"