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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - FEN1 antibody (ab2619)
Predicted band size : 43 kDa
ab2619 at a 1:2500 dilution staining FEN 1 in A) Raji whole cell lysate and B) NIH-3T3 whole cell lysate by Western Blot (ECL)
Western blot - FEN 1 antibody (ab2619)
All lanes : Anti-FEN1 antibody (ab2619) at 1/2500 dilution
Lane 1 : Soluble
Lane 2 : Unbound
Lane 3 : Wash
Lane 4 : 250mM Imidazole treated: Eluate 1
Lane 5 : 250mM Imidazole treated: Eluate 2
Lane 6 : 250mM Imidazole treated: Eluate 3
Lane 7 : 250mM Imidazole treated: Eluate 4
Lane 8 : 250mM Imidazole treated: Eluate 5
Lane 9 : 250mM Imidazole treated: Eluate 6
Lane 10 : 250mM Imidazole treated: Eluate 7
Lane 11 : 250mM Imidazole treated: Eluate 8
Secondary
HRP conjugated goat anti-rabbit IgG
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 seconds
Ab 2619 used in a Western blot of recombinant protein purified from SF9 cells. Lanes 4 - 11 represent eluates from imidazole treated cells.
This image is courtesy of an Abreview submitted on 16 March 2006.
FEN 1 antibody for WB in Human (2619)
Anti-FEN1 antibody for Western blot in Mouse (2619)
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