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Western blot - FEN1 antibody (ab2619)
(enlarge)
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Western blot - FEN 1 antibody (ab2619)
(enlarge)
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Product Name
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FEN1 antibody
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See all FEN1 antibodies (12)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to FEN1
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Immunogen
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Recombinant human FEN 1 protein purified from E.coli.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/2500. Detects a band of approximately 52 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa, Raji or 3T3 whole cell lysates
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
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Relevance
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FEN 1 (Flap Endonuclease and Five prime Exonuclease 1) functions as an endonuclease when it cleaves branched DNA structures containing single stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN 1 endonuclease and exonuclease activities are stimulated by an association with Proliferating Cell Nuclear Antigen (PCNA) in the nucleus. FEN 1 is believed to have important roles in DNA replication and repair and is required in cell free DNA replication systems.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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unknown
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FEN1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for FEN1 antibody images, references, products related to ab2619 and other tools.
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FEN1 antibody images:
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 Western blot - FEN1 antibody (ab2619)
Predicted band size : 43 kDa
ab2619 at a 1:2500 dilution staining FEN 1 in A) Raji whole cell lysate and B) NIH-3T3 whole cell lysate by Western Blot (ECL)
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Western blot - FEN 1 antibody (ab2619)
All lanes : FEN1 antibody (ab2619) at 1/2500 dilution
Lane 1 : Soluble
Lane 2 : Unbound
Lane 3 : Wash
Lane 4 : 250mM Imidazole treated: Eluate 1
Lane 5 : 250mM Imidazole treated: Eluate 2
Lane 6 : 250mM Imidazole treated: Eluate 3
Lane 7 : 250mM Imidazole treated: Eluate 4
Lane 8 : 250mM Imidazole treated: Eluate 5
Lane 9 : 250mM Imidazole treated: Eluate 6
Lane 10 : 250mM Imidazole treated: Eluate 7
Lane 11 : 250mM Imidazole treated: Eluate 8
Secondary
HRP conjugated goat anti-rabbit IgG
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 seconds
Ab 2619 used in a Western blot of recombinant protein purified from SF9 cells. Lanes 4 - 11 represent eluates from imidazole treated cells. This image is courtesy of an Abreview submitted on 16 March 2006.
See Abreview
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References for FEN1 antibody (ab2619)
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This product has been used in:
Kalifa L et al. Evidence for a role of FEN1 in maintaining mitochondrial DNA integrity. DNA Repair (Amst) 8:1242-9 (2009). WB; Mouse. PubMed: 19699691
If you publish research using ab2619 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to FEN1
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FEN1 antibody - more information
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Customer reviews (feedback) regarding FEN1 antibody |
Customer FAQs regarding FEN1 antibody |
Protocols for FEN1 antibody |
Price and availability of products related to FEN1 antibody |
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FEN1 antibody - related products:
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FEN1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about FEN1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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