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ab73361 |
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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Anti-FEN1 antibody
See all FEN1 products (14) ...
Rabbit polyclonal to FEN1
Reacts with
Mouse, Rat
Predicted to work with
Cow, Human
Synthetic peptide derived from within residues 100 - 200 of Human FEN1.
(Peptide available as ab73361.)
This antibody gave a positive signal in the following tissue lysates: Mouse Liver, Mouse Kidney, Rat Kidney
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Western blot - FEN1 antibody (ab73362)
(enlarge)
Our Abpromise guarantee covers the use of ab73362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.
Target information above from: UniProt accessionP39748
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - FEN1 antibody (ab73362)

All lanes : Anti-FEN1 antibody (ab73362) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 43 kDa
Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
ab73362 has not yet been referenced specifically in any publications.
Publishing research using ab73362? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:

All lanes : Anti-FEN1 antibody (ab73362) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 43 kDa
Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
1
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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