|
|
|
|
Western blot - FEN1 antibody (ab73362)
(enlarge)
|
|
|
|
|
Product Name
|
FEN1 antibody
|
| |
See all FEN1 antibodies (12)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to FEN1
|
|
Immunogen
|
Synthetic peptide derived from within residues 100 - 200 of Human FEN1. (Peptide available as ab73361.)
|
|
Reacts with
|
Ms, Rat
|
|
Predicted to react
(species key)
|
Hu, Cow
(Due to sequence homology)
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
This antibody gave a positive signal in the following tissue lysates: Mouse Liver, Mouse Kidney, Rat Kidney
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
|
|
Relevance
|
FEN 1 (Flap Endonuclease and Five prime Exonuclease 1) functions as an endonuclease when it cleaves branched DNA structures containing single stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN 1 endonuclease and exonuclease activities are stimulated by an association with Proliferating Cell Nuclear Antigen (PCNA) in the nucleus. FEN 1 is believed to have important roles in DNA replication and repair and is required in cell free DNA replication systems.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FEN1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for FEN1 antibody images, references, products related to ab73362 and other tools.
|
| |
FEN1 antibody images:
|
 Western blot - FEN1 antibody (ab73362)
All lanes : FEN1 antibody (ab73362) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 43 kDa
Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
|
| |
| |
|
Search PubMed (MEDLINE) for references to FEN1
|
| |
FEN1 antibody - more information
|
Customer reviews (feedback) regarding FEN1 antibody |
Customer FAQs regarding FEN1 antibody |
Protocols for FEN1 antibody |
Price and availability of products related to FEN1 antibody |
| |
FEN1 antibody - related products:
|
|
|
| |
FEN1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about FEN1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
