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- Secondary antibodies
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Product name
Anti-FER antibody - C-terminal
See all FER products (8) ...
Description
Rabbit polyclonal to FER - C-terminal
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the C-terminal region of human FER conjugated to KLH.
Positive control
HL-60 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab71682 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Western blot - FER antibody - C-terminal (ab71682)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).
Target
Function
Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
Tissue specificity
Expressed in a variety of lymphoid cell lines.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Cellular localization
Cytoplasm. Nucleus. Associated with the chromatin.
Target information above from: UniProt accessionP16591
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- c FER antibody
- fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
- Fer (fps/fes related) tyrosine kinase antibody
see all
Database links
- Entrez Gene: 2241 Human
- NCBI: 4885231 Human
- Omim: 176942 Human
- SwissProt: P16591 Human
- Unigene: 221472 Human
Anti-FER antibody - C-terminal images:
Western blot - FER antibody - C-terminal (ab71682)
Anti-FER antibody - C-terminal (ab71682) at 1/100 dilution + HL-60 cell lysate at 12.5 µg
Predicted band size : 93 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa,75 kDa. We are unsure as to the identity of these extra bands.
References for Anti-FER antibody - C-terminal (ab71682)
This product has been referenced in:
- Zoubeidi A et al. The Fer tyrosine kinase cooperates with interleukin-6 to activate signal transducer and activator of transcription 3 and promote human prostate cancer cell growth. Mol Cancer Res 7:142-55 (2009).Read more (PubMed: 19147545) »
See 1 publication for this product
Publishing research using ab71682? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"