![Western blot - FER antibody [EP1842Y] (ab52479) image](#ab52479.jpg)
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Product name
Anti-FER antibody [EP1842Y]
See all FER products (8) ...
Description
Rabbit monoclonal [EP1842Y] to FER
Specificity
This antibody reacts with FER
Tested applications
WB, IP, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues on the N terminus of FER (Human)
Positive control
Jurkat cell lysate
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1842Y
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Western blot - FER antibody [EP1842Y] (ab52479)
![Western blot - FER antibody [EP1842Y] (ab52479) image](/ps/datasheet/Images/52/ab52479/ab52479.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
IP
IP: 1/30.IP: 1/30.
Flow Cyt
Flow Cyt: 1/50.Flow Cyt: 1/50.
Application notes
Is unsuitable for ,ICC or IHC-P.
Target
Function
Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
Tissue specificity
Expressed in a variety of lymphoid cell lines.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Cellular localization
Cytoplasm. Nucleus. Associated with the chromatin.
Target information above from: UniProt accessionP16591
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- c FER antibody
- Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
- Fer (fps/fes related) tyrosine kinase antibody
see all
Database links
- Entrez Gene: 2241 Human
- Entrez Gene: 14158 Mouse
- NCBI: 4885231 Human
- Omim: 176942 Human
- SwissProt: P16591 Human
- SwissProt: P70451 Mouse
- SwissProt: P09760 Rat
- Unigene: 221472 Human
see all
Anti-FER antibody [EP1842Y] images:
Western blot - FER antibody [EP1842Y] (ab52479)
Anti-FER antibody [EP1842Y] (ab52479) at 1/5000 dilution + Jurkat cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 93 kDa
Observed band size : 93 kDa
References for Anti-FER antibody [EP1842Y] (ab52479)
This product has been referenced in:
- McGinnis LK et al. Fer tyrosine kinase is required for germinal vesicle breakdown and meiosis-I in mouse oocytes. Mol Reprod Dev 78:33-47 (2011). IHC-P, IF; Mouse.Read more (PubMed: 21268181) »
- Whitehead SN et al. Transient and bilateral increase in Neuropilin-1, Fer kinase and collapsin response mediator proteins within membrane rafts following unilateral occlusion of the middle cerebral artery in mouse. Brain Res 1344:209-16 (2010). WB; Mouse.Read more (PubMed: 20493826) »
See all 3 publications for this product
Publishing research using ab52479? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"