![Western blot - FER antibody [Not given] (ab109740) image](#FER-Primary-antibodies-ab109740-1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER antibody [Not given] (ab109740) image](#FER-Primary-antibodies-ab109740-2.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER antibody [Not given] (ab109740) image](#FER-Primary-antibodies-ab109740-3.jpg)
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Product name
Anti-FER antibody [5D2C4]
See all FER products (8) ...
Description
Mouse monoclonal [5D2C4] to FER
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Ni-NTA purified truncated recombinant FER-His expressed in E. Coli strain BL21 (DE3).
Positive control
Truncated FER-His recombinat protein, Human Kidney, Human Spleen
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
5D2C4
Isotype
IgG1
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab109740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/2000. Predicted molecular weight: 95 kDa.
ELISA
ELISA: 1/10000.ELISA: 1/10000.
IHC-P: 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
Tissue specificity
Expressed in a variety of lymphoid cell lines.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Cellular localization
Cytoplasm. Nucleus. Associated with the chromatin.
Target information above from: UniProt accessionP16591
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- c FER antibody
- Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
- Fer (fps/fes related) tyrosine kinase antibody
see all
Database links
- Entrez Gene: 2241 Human
- NCBI: 4885231 Human
- Omim: 176942 Human
- SwissProt: P16591 Human
- Unigene: 221472 Human
Anti-FER antibody [5D2C4] images:
Western blot - FER antibody [Not given] (ab109740)
![Western blot - FER antibody [Not given] (ab109740)](/ps/datasheet/images/109/ab109740/FER-Primary-antibodies-ab109740-1.jpg)
All lanes : Anti-FER antibody [5D2C4] (ab109740) at 1/500 dilution
Lane 1 : Truncated FER-His recombinant protein
Lane 2 : NIH3T3 cell lysate
Predicted band size : 95 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER antibody [Not given] (ab109740)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER antibody [Not given] (ab109740)](/ps/datasheet/images/109/ab109740/FER-Primary-antibodies-ab109740-2.jpg)
ab109740 at 1/200 dilution staining FER in Human Kidney by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER antibody [Not given] (ab109740)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER antibody [Not given] (ab109740)](/ps/datasheet/images/109/ab109740/FER-Primary-antibodies-ab109740-3.jpg)
ab109740 at 1/200 dilution staining FER in human spleen by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
References for Anti-FER antibody [5D2C4] (ab109740)
ab109740 has not yet been referenced specifically in any publications.
Publishing research using ab109740? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"