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Product name
Anti-FER antibody
See all FER products (8) ...
Description
Rabbit polyclonal to FER
Specificity
Detects endogenous levels of total FER protein.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthesized non phosphopeptide derived from human FER around the phosphorylation site of tyrosine 402 (V-N-YP-E-E).
Positive control
Extracts from HeLa cells, treated with paclitaxel (1 µM, 24 hours).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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Western blot - FER antibody (ab79358)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab79358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Predicted molecular weight: 93 kDa.
ELISA
ELISA: 1/40000.ELISA: 1/40000.
Target
Function
Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
Tissue specificity
Expressed in a variety of lymphoid cell lines.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Cellular localization
Cytoplasm. Nucleus. Associated with the chromatin.
Target information above from: UniProt accessionP16591
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- c FER antibody
- Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
- Fer (fps/fes related) tyrosine kinase antibody
see all
Database links
- Entrez Gene: 2241 Human
- Entrez Gene: 14158 Mouse
- NCBI: 4885231 Human
- Omim: 176942 Human
- SwissProt: P16591 Human
- SwissProt: P70451 Mouse
- Unigene: 221472 Human
- Unigene: 23039 Mouse
Anti-FER antibody images:
Western blot - FER antibody (ab79358)
All lanes : Anti-FER antibody (ab79358) at 1/500 dilution
Lane 1 : extracts from HeLa cells, treated
with paclitaxel (1 µM, 24 hours)
Lane 2 : extracts from HeLa cells, treated
with paclitaxel (1 µM, 24 hours) with immunizing peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size : 93 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa,70 kDa. We are unsure as to the identity of these extra bands.
References for Anti-FER antibody (ab79358)
ab79358 has not yet been referenced specifically in any publications.
Publishing research using ab79358? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"