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Anti-FER (phospho Y402) antibody (ab79573)

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Overview

Product name

Anti-FER (phospho Y402) antibody
See all FER products (8) ...

Description

Rabbit polyclonal to FER (phospho Y402)

Specificity

ab79573 detects endogenous levels of FER only when phosphorylated at tyrosine 402.

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse

Immunogen

Synthetic phosphopeptide derived from human FER around the phosphorylation site of tyrosine 402 (V-N-YP-E-E)

Positive control

Human brain tissue; Jurkat cell extracts starved for 24 hours.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab79573 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER (phospho Y402) antibody (ab79573)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER (phospho Y402) antibody (ab79573) image (enlarge)

  • Western blot - FER (phospho Y402) antibody (ab79573)Western blot - FER (phospho Y402) antibody (ab79573) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab79573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000. Pred...Read more →

    WB: 1/500 - 1/1000. Predicted molecular weight: 93 kDa.

  • ELISA

     ELISA: 1/20000.

    ELISA: 1/20000.

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Function

Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.

Tissue specificity

Expressed in a variety of lymphoid cell lines.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.

Cellular localization

Cytoplasm. Nucleus. Associated with the chromatin.

Target information above from: UniProt accessionP16591 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • c FER antibody
  • Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
  • Fer (fps/fes related) tyrosine kinase antibody
  • FER antibody
  • FER_HUMAN antibody
  • p94 FER antibody
  • p94-FER antibody
  • Phosphoprotein NCP94 antibody
  • Proto oncogene tyrosine protein kinase FER antibody
  • Proto-oncogene c-Fer antibody
  • TYK3 antibody
  • Tyrosine kinase 3 antibody
  • Tyrosine-protein kinase Fer antibody
see all

Database links

Anti-FER (phospho Y402) antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER (phospho Y402) antibody (ab79573)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FER (phospho Y402) antibody (ab79573)

ab79573, at a 1/50 dilution, staining FER in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.

  Western blot - FER (phospho Y402) antibody (ab79573)

Western blot - FER (phospho Y402) antibody (ab79573)

All lanes : Anti-FER (phospho Y402) antibody (ab79573) at 1/500 dilution

Lane 1 : Jurkat cell extracts, starved (24 hours)
Lane 2 : Jurkat cell extracts, starved (24 hours) with immunizing phospho peptide at 10 µg

Lysates/proteins at 30 µg per lane.


Predicted band size : 93 kDa
Observed band size : 93 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

References for Anti-FER (phospho Y402) antibody (ab79573)

ab79573 has not yet been referenced specifically in any publications.

Publishing research using ab79573? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"