FGF-21 Human ELISA Kit (ab125966)
- Product nameFGF-21 Human ELISA Kit
Intra-assay Sample n Mean SD CV% Overall 4.8%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeCompetitive
- Sensitivity0.03 ng/ml
- Range0.031 ng/ml - 2 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Fibroblast growth factor 21 (FGF21) is a member of endocrine FGF subfamily, along with FGF19 and FGF23. The secreted Human FGF21 is expressed in liver, pancreas, and white adipose tissue. It contains 209 amino acids in the precursor and 181 amino acids in the mature protein with a molecular mass of about 20 kDa, and has 75% homology with mouse FGF-21. FGF21 signals through cell-surface tyrosine kinase FGF receptors complexed with a cofactor ß-Klotho. FGF21 is a novel metabolic regulator involved in glucose metabolism, lipolysis, and ketogenesis and triglyceride clearance, growth hormone signaling, and metabolic diseases. In rodent models of diabetes, it stimulates glucose uptake in adipocytes, protects animals from dietinduced obesity, and lowers blood glucose and triglyceride. Serum FGF21 levels are increased in patients with metabolic diseases including nonalcoholic fatty liver disease, type 2 diabetes, gestational diabetes, obesity, Cushing’s syndrome, HIV-1-induced lipodystrophy, and chronic kidney hemodialysis. Conversely, circulating FGF21 concentrations were reduced in subjects with anorexia nervosa. FGF21 is a biomarker for metabolic diseases and a candidate for the treatment of insulin resistance.
ab125966 FGF-21 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human FGF21 in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures FGF21 in less than 5 hours. A polyclonal antibody specific for FGF21 has been pre-coated onto a microplate. FGF21 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for FGF21, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Human FGF-21 Antibody (100x) 1 x 80µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human FGF-21 Microplate 1 x 96 tests Human FGF-21 Standard (lyophilized) 1 vial Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml
- Research Areas
- FGF 21FGF-21Fgf21
- FGF21_HUMANFibroblast growth factor 21
Our Abpromise guarantee covers the use of ab125966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for FGF-21 Human ELISA Kit (ab125966)
ab125966 has not yet been referenced specifically in any publications.