Overview

  • Product nameFGF-21 Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Overall 4.7%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.03 ng/ml
  • Range
    0.031 ng/ml - 2 ng/ml
  • Recovery

    97 %

  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s FGF-21 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human FGF-21 in plasma, serum, and cell culture supernatants.

    A FGF-21 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a FGF-21 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of FGF-21 captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionStimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • FGF 21
    • FGF-21
    • Fgf21
    • FGF21_HUMAN
    • Fibroblast growth factor 21
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab125966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

FGF-21 Human ELISA Kit images

  • FGF-21 measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab125966

Protocols

References for FGF-21 Human ELISA Kit (ab125966)

ab125966 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"