Overview
- Product nameAnti-FGF1 antibody (Biotin)See all FGF1 primary antibodies ...
- DescriptionRabbit polyclonal to FGF1 (Biotin)
- ConjugationBiotin
- Tested applicationsELISA, Sandwich ELISA, WB more details
- Species reactivityReacts with: Human
- Immunogen
Highly pure (>98%) recombinant human FGF acidic
Properties
- FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Purification notesab83163 is sterile filtered.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab83163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | |
| Sandwich ELISA | |
| WB |
sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human FGF acidic.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human FGF acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 17 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
- Sequence similaritiesBelongs to the heparin-binding growth factors family.
- Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
-
Database links
- Entrez Gene: 2246 Human
- Omim: 131220 Human
- SwissProt: P05230 Human
- Unigene: 483635 Human
Target information above from: UniProt accession
P05230
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- Acidic fibroblast growth factor antibodyAFGF antibodyBeta endothelial cell growth factor antibody
- Beta-endothelial cell growth factor antibodyECGF antibodyECGF beta antibodyECGF-beta antibodyECGFA antibodyECGFB antibodyEndothelial Cell Growth Factor alpha antibodyEndothelial Cell Growth Factor beta antibodyFGF 1 antibodyFGF alpha antibodyFgf1 antibodyFGF1_HUMAN antibodyFGFA antibodyFibroblast Growth Factor 1 Acidic antibodyFibroblast growth factor 1 antibodyGLIO703 antibodyHBGF 1 antibodyHBGF-1 antibodyHBGF1 antibodyHeparin binding growth factor 1 antibodyHeparin binding growth factor 1 precursor antibodyHeparin-binding growth factor 1 antibody
see all
References for Anti-FGF1 antibody (Biotin) (ab83163)
ab83163 has not yet been referenced specifically in any publications.
