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Overview

  • Product nameFGF basic Mouse ELISA KitSee all FGF basic kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 8 pg/ml
  • Range
    18.8 pg/ml - 600 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 119.7 105% - 129%
    Serum 84.63 78% - 100%
    Plasma 83.26 76% - 99%

  • Species reactivity
    Reacts with: Mouse
  • Product overview

     

    Abcam’s Fibroblast growth factor (FGF2) basic mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse FGF basic in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    FGF basic is a member of the fibroblast growth factor family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

  • Tested applicationsELISA more details

Properties

  • RelevanceFGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
    • Alternative names
        Basic fibroblast growth factor BFGF FGF 2
        FGF B FGF2 FGF2 basic FGFBFibroblast growth factor 2 (basic) HBGF 2HBGF2HBGH 2Heparin binding growth factor 2Heparin binding growth factor 2 precursor OTTMUSP00000046776Prostatropin
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100670 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      FGF basic Mouse ELISA Kit images

      • TYPICAL STANDARD CURVE- Assay Diluent B
        TYPICAL STANDARD CURVE- Assay Diluent B
        TYPICAL STANDARD CURVE- Assay Diluent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
      • TYPICAL STANDARD CURVE- Assay Diluent A
        TYPICAL STANDARD CURVE- Assay Diluent A
        TYPICAL STANDARD CURVE- Assay Diluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

      Protocols

      References for FGF basic Mouse ELISA Kit (ab100670)

      ab100670 has not yet been referenced specifically in any publications.

      Publishing research using ab100670 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100670 Submit a Question for ab100670

      Displaying 1 - 5 of 5 results for Abreviews and Q&A

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      Dear Sir/Madam,

      I would like to use cell-lysates in your FGF basic mouse ELISA kit (ab100670). In order to make these lysates we use either Tris-buffer or Ripa-buffer. Do you know if these buffer may influence the measurement of the ELISA ? And...

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      Thank you for your email.

      I assume you are looking for a lysis buffer because you wish to test cell or tissue lysates samples with the kit? If so, please note that the ab100670 kit has not been validated with lysate samples, only serum, plasma, ...

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      I would be very grateful if you could tell me if we can use the ELISA kit for FGF2 to measure the levels in brain homogenates.

      Thank you for contacting us.

      Although this kit was neither designed nor tested with cell or tissue lysate samples, this kit as well as our other sandwich ELISA kits are very accommodating to many different sample types so lysates samples should ...

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      I ordered a CNTF Rat ELISA kit three months ago. I would like to order other ELISA kits, for example BDNF Rat ELISA kit, GDNF Rat ELISA kit and FGF Rat ELISA kit. I do not find them on abcam's webpage. Could you send me some information about these produc...

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      Thank you for contacting us. We do not currently sell any ELISA kits that are specifically designed for use on rat samples. After checking with the lab, our mouse and human ELISA kits for these targets will be unlikely to cross-react with rat, but have no...

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      To whom it may concern,
      I would like to have some information about the specificity of the hu and mu FGF2 ELISA kit (ab 99980 and ab100670)
      Given the high similarity between the human and mouse bFGF sequence, we had often experience of crossreac...

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      Thank you very much for contacting us.

      Unfortunately, epitope mapping of the two antibody pairs has not been performed, so we do not know the exact amino acid sequence recognised and if these regions are specific for human and mouse, respective...

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      Should there be a blocking step in the protocol?

      Thank you for your phone call today. I have heard back from my contact at the laboratory, and I was told that the ELISA plates come pre-blocked so a blocking step is not necessary.  I hope this information is helpful, but please let me know if you ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"