FGF basic Mouse ELISA Kit (ab100670)
- Product nameFGF basic Mouse ELISA KitSee all FGF basic kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 8 pg/ml
- Range18.8 pg/ml - 600 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 119.7 105% - 129% Serum 84.63 78% - 100% Plasma 83.26 76% - 99%
- Species reactivityReacts with: Mouse
- Product overview
Abcam’s Fibroblast growth factor (FGF2) basic mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse FGF basic in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
FGF basic is a member of the fibroblast growth factor family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 300X HRP-Streptavidin Concentrate Item G 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse FGF basic 2 vials FGF basic Microplate 1 unit Mouse bFGF Standard (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Basic fibroblast growth factor BFGF FGF 2
- FGF B FGF2 FGF2 basic FGFBFibroblast growth factor 2 (basic) HBGF 2HBGF2HBGH 2Heparin binding growth factor 2Heparin binding growth factor 2 precursor OTTMUSP00000046776Prostatropin
Our Abpromise guarantee covers the use of ab100670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FGF basic Mouse ELISA Kit images
TYPICAL STANDARD CURVE- Assay Diluent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
TYPICAL STANDARD CURVE- Assay Diluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
References for FGF basic Mouse ELISA Kit (ab100670)
ab100670 has not yet been referenced specifically in any publications.