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Product name
Anti-FGF basic antibody [0.T.50] - Azide free
See all FGF basic products (27) ...
Description
Mouse monoclonal [0.T.50] to FGF basic - Azide free
Specificity
ab33103 recognizes the native, non denatured form of basic FGF. It does not recognise acidic FGF (HBGF 1).
Tested applications
Neutralisingmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Full length native protein: Purified bovine brain basic FGF (HBGF-2).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 40% Glycerol, Tris buffered saline
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
ab33103 was purified by ammonium sulfate precipitation, Sephacryl S-300 gel filtration and DEAE-52 chromatography.
Clonality
Monoclonal
Clone number
0.T.50
Isotype
IgG1
Light chain type
kappa
Research areas
Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> FGF
Stem Cells >> Neural Stem Cells >> Extracellular
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Neut: Use a concentration of 2.5 µg/ml.
Application notes
Is unsuitable for or WB.
Target
Function
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
Tissue specificity
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
Sequence similarities
Belongs to the heparin-binding growth factors family.
Post-translational
modifications
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
Cellular localization
Secreted. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane.
Target information above from: UniProt accessionP09038
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Basic fibroblast growth factor antibody
- Basic fibroblast growth factor antibody
- BFGF antibody
see all
Database links
- Entrez Gene: 281161 Cow
- Entrez Gene: 2247 Human
- Entrez Gene: 14173 Mouse
- Entrez Gene: 54250 Rat
- Omim: 134920 Human
- SwissProt: P03969 Cow
- SwissProt: P09038 Human
- SwissProt: P15655 Mouse
see all
References for Anti-FGF basic antibody [0.T.50] - Azide free (ab33103)
This product has been referenced in:
- Kujawski M et al. Stat3 mediates myeloid cell-dependent tumor angiogenesis in mice. J Clin Invest 118:3367-77 (2008). Neutralising; Human.Read more (PubMed: 18776941) »
See 1 publication for this product
Publishing research using ab33103? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"