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Anti-FGF basic antibody [AS24] (ab17279)

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Overview

Product name

Anti-FGF basic antibody [AS24]
See all FGF basic products (27) ...

Description

Mouse monoclonal [AS24] to FGF basic

Specificity

Recognises human basic FGF, does not recognise human acidic FGF or a panel of other human cytokines.

Tested applications

IHC-Fr, Sandwich ELISA, ELISAmore details

Cross reactivity

Reacts with

Human

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.08% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

AS24

Isotype

IgG1

Research areas

Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> FGF
Stem Cells >> Neural Stem Cells >> Extracellular
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF

  • Sandwich ELISA - FGF basic antibody [AS24] (ab17279)Sandwich ELISA - FGF basic antibody [AS24] (ab17279) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab17279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent dilution. This antibody can be used at 1-10 ug/ml to stain human basic FGF expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10 minutes at 4°C, plus 0.1% saponin for 20minutes at room temperature.

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    Sandwich ELISA

      See more...Read more →

    sELISA: Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit monoclonal [EP1735] to FGF basic (ab92337). For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit monoclonal [EP1735] to FGF basic (ab92337) as Detection.

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    ELISA

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    ELISA: Use at an assay dependent dilution. This antibody can be used as a coating antibody at 10 µg/ml in a two site ELISA with biotin conjugated bFGF antibody. Typically the ELISA has a working range of 0-2000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of human basic FGF in human serum is typically > 85% in the ELISA.

Target

Function

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.

Tissue specificity

Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

Sequence similarities

Belongs to the heparin-binding growth factors family.

Post-translational
modifications

Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

Cellular localization

Secreted. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane.

Target information above from: UniProt accessionP09038 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Basic fibroblast growth factor antibody
  • Basic fibroblast growth factor antibody
  • BFGF antibody
  • BFGF antibody
  • FGF 2 antibody
  • FGF B antibody
  • FGF-2 antibody
  • FGF2 antibody
  • FGF2 basic antibody
  • FGF2_HUMAN antibody
  • FGFB antibody
  • FGFB antibody
  • Fibroblast growth factor 2 (basic) antibody
  • Fibroblast growth factor 2 (basic) antibody
  • Fibroblast growth factor 2 antibody
  • HBGF 2 antibody
  • HBGF-2 antibody
  • HBGF2 antibody
  • HBGF2 antibody
  • HBGH 2 antibody
  • HBGH2 antibody
  • HBGH2 antibody
  • Heparin binding growth factor 2 precursor antibody
  • Heparin binding growth factor 2 precursor antibody
  • Heparin-binding growth factor 2 antibody
  • Prostatropin antibody
  • Prostatropin antibody
see all

Database links

Anti-FGF basic antibody [AS24] images:

  Sandwich ELISA - FGF basic antibody [AS24] (ab17279)

Sandwich ELISA - FGF basic antibody [AS24] (ab17279)

Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1ug/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5ug/ml.

Protocols

  • Protocol Booklet

    • References for Anti-FGF basic antibody [AS24] (ab17279)

    ab17279 has not yet been referenced specifically in any publications.

    Publishing research using ab17279? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"