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Product name
Anti-FGF basic antibody [AS25] (Biotin)
See all FGF basic products (27) ...
Description
Mouse monoclonal [AS25] to FGF basic (Biotin)
Conjugation
Biotin
Specificity
Does not recognise human acidic FGF or a panel of other human cytokines
Tested applications
ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Unfortunately, this information is considered to be commercially sensitive
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.08% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Antibody Purity > 95 % by SDS PAGE
Clonality
Monoclonal
Clone number
AS25
Isotype
IgG1
Research areas
Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> FGF
Stem Cells >> Neural Stem Cells >> Extracellular
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
Application notes
ELISA: This antibody can be used as a solution phase antibody at 2 µg/ml in a two site ELISA with unconjugated FGF basic antibody. Typically the ELISA has a working range of 0-2000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recovery of human basic FBF is human serum is typically >85% in the ELISA.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
Tissue specificity
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
Sequence similarities
Belongs to the heparin-binding growth factors family.
Post-translational
modifications
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
Cellular localization
Secreted. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane.
Target information above from: UniProt accessionP09038
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Basic fibroblast growth factor antibody
- BFGF antibody
- FGF 2 antibody
see all
Database links
- Entrez Gene: 2247 Human
- Omim: 134920 Human
- SwissProt: P09038 Human
- Unigene: 284244 Human
References for Anti-FGF basic antibody [AS25] (Biotin) (ab16167)
ab16167 has not yet been referenced specifically in any publications.
Publishing research using ab16167? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"