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Anti-FGF basic antibody - Aminoterminal end (ab65973)

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Overview

Product name

Anti-FGF basic antibody - Aminoterminal end
See all FGF basic products (27) ...

Description

Rabbit polyclonal to FGF basic - Aminoterminal end

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide from near the N-terminal of human FGF basic, identical to the related rat and mouse sequence

Positive control

Rat lung tissue lysate and mammary cancer sections

Properties

Form

Lyophilised:
0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> FGF
Stem Cells >> Neural Stem Cells >> Extracellular
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF basic antibody (ab65973)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF basic antibody (ab65973) image (enlarge)

  • Western blot - FGF basic antibody - Aminoterminal end (ab65973)Western blot - FGF basic antibody - Aminoterminal end (ab65973) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab65973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

  • ShowHide1 Image

    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 1 - 2 µg/ml.

Target

Function

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.

Tissue specificity

Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

Sequence similarities

Belongs to the heparin-binding growth factors family.

Post-translational
modifications

Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

Cellular localization

Secreted. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane.

Target information above from: UniProt accessionP09038 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Basic fibroblast growth factor antibody
  • BFGF antibody
  • FGF 2 antibody
  • FGF B antibody
  • FGF-2 antibody
  • FGF2 antibody
  • FGF2 basic antibody
  • FGF2_HUMAN antibody
  • FGFB antibody
  • Fibroblast growth factor 2 (basic) antibody
  • Fibroblast growth factor 2 antibody
  • HBGF 2 antibody
  • HBGF-2 antibody
  • HBGF2 antibody
  • HBGH 2 antibody
  • HBGH2 antibody
  • Heparin binding growth factor 2 precursor antibody
  • Heparin-binding growth factor 2 antibody
  • Prostatropin antibody
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Database links

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Anti-FGF basic antibody - Aminoterminal end images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF basic antibody (ab65973)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF basic antibody (ab65973)

ab65973 at 1µg/ml staining FGF basic in human intestinal cancer tissue sections.

  Western blot - FGF basic antibody - Aminoterminal end (ab65973)

Western blot - FGF basic antibody - Aminoterminal end (ab65973)

Anti-FGF basic antibody - Aminoterminal end (ab65973) at 1 µg/ml + Rat lung tissue lysis at 30 µg

Predicted band size : 17 kDa
Observed band size : 17 kDa

References for Anti-FGF basic antibody - Aminoterminal end (ab65973)

This product has been referenced in:

  • Paiva P  et al. Human chorionic gonadotrophin regulates FGF2 and other cytokines produced by human endometrial epithelial cells, providing a mechanism for enhancing endometrial receptivity. Hum Reprod 26:1153-62 (2011). IHC-P; Human.Read more (PubMed: 21345913) »
  • Huang JJ  et al. Eotaxin and FGF enhance signaling through an extracellular signal-related kinase (ERK)-dependent pathway in the pathogenesis of Eosinophilic esophagitis. Allergy Asthma Clin Immunol 6:25 (2010). IHC-P; Human.Read more (PubMed: 20815913) »

See all 3 publications for this product

Publishing research using ab65973? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"