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Anti-FGF basic antibody [EP1735] (ab92337)

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Overview

Product name

Anti-FGF basic antibody [EP1735]
See all FGF basic products (27) ...

Description

Rabbit monoclonal [EP1735] to FGF basic

Tested applications

WB, IP, Flow Cyt, Sandwich ELISAmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human FGF basic.

Positive control

K562 cell lysate.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1735

Isotype

IgG

Research areas

Developmental Biology >> Organogenesis >> Excretory system development >> Kidney development
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> FGF
Stem Cells >> Neural Stem Cells >> Extracellular
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF

  • Sandwich ELISA - FGF basic antibody [EP1735] (ab92337)Sandwich ELISA - FGF basic antibody [EP1735] (ab92337) image (enlarge)

  • Western blot - FGF basic antibody [EP1735] (ab92337)Western blot - FGF basic antibody [EP1735] (ab92337) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab92337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for or IHC-P.

Target

Function

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.

Tissue specificity

Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

Sequence similarities

Belongs to the heparin-binding growth factors family.

Post-translational
modifications

Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

Cellular localization

Secreted. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane.

Target information above from: UniProt accessionP09038 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Basic fibroblast growth factor antibody
  • BFGF antibody
  • FGF 2 antibody
  • FGF B antibody
  • FGF-2 antibody
  • FGF2 antibody
  • FGF2 basic antibody
  • FGF2_HUMAN antibody
  • FGFB antibody
  • Fibroblast growth factor 2 (basic) antibody
  • Fibroblast growth factor 2 antibody
  • HBGF 2 antibody
  • HBGF-2 antibody
  • HBGF2 antibody
  • HBGH 2 antibody
  • HBGH2 antibody
  • Heparin binding growth factor 2 precursor antibody
  • Heparin-binding growth factor 2 antibody
  • Prostatropin antibody
see all

Database links

Anti-FGF basic antibody [EP1735] images:

  Sandwich ELISA - FGF basic antibody [EP1735] (ab92337)

Sandwich ELISA - FGF basic antibody [EP1735] (ab92337)

Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1ug/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5ug/ml.

  Western blot - FGF basic antibody [EP1735] (ab92337)

Western blot - FGF basic antibody [EP1735] (ab92337)

Anti-FGF basic antibody [EP1735] (ab92337) at 1/1000 dilution + K562 cell lysate at 10 µg

Secondary
goat anti-rabbit HRP at 1/2000 dilution

Predicted band size : 31 kDa

Protocols

  • Protocol Booklet

    • References for Anti-FGF basic antibody [EP1735] (ab92337)

    ab92337 has not yet been referenced specifically in any publications.

    Publishing research using ab92337? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"