Overview
Product nameFGF basic protein (Active)See all FGF basic proteins and peptides ...
Protein descriptionFull length recombinant human FGF basic produced in Sf9 insect cells (using baculovirus expression system). 155 amino acids. M.wt 17kDa. Swiss-Prot P09038.
Expression hostInsect cells
Properties
Purity> 95
% by SDS-PAGE
Purification notesThis protein was purified by proprietary chromatographic techniques.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological activitySpecific activity of 2 x 106 Units/mg.
FormLiquid
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: None
Constituents: 20% Glycerol, 20mM Tris, 100mM Potassium chloride, 1mM DTT, pH 7.9
Concentration information loading...
Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Sequence notesThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ala-Gly-Ser-Ile.
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab73134
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
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Notes |
| SDS-PAGE |
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| Functional Studies |
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Application notesBiological Activity: the ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is 0.5 ng/ml.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
Basic fibroblast growth factorBasic fibroblast growth factor bFGFBFGF
FGF 2FGF BFGF-2FGF2FGF2 basicFGF2_HUMANFGFBFibroblast growth factor 2Fibroblast growth factor 2 (basic)HBGF 2HBGF-2HBGF2HBGH 2HBGH2Heparin binding growth factor 2 precursorHeparin-binding growth factor 2Prostatropin
see all
FunctionPlays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
Tissue specificityExpressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
Post-translational
modificationsPhosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
Cellular localizationSecreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
References for FGF basic protein (Active) (ab73134)
ab73134
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
