Overview
Product nameFGF basic proteinSee all FGF basic proteins and peptides ...
Protein descriptionRecombinant fragment (Mouse); a 16.2 kDa protein consisting of 145 amino acid residues.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab50235 purity is greater than 98% by SDS-PAGE and HPLC analyses.
FormLyophilised:Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. Repeated freeze thaw cycles will result in loss of activity.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Concentration information loading...
Sequence notesMPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab50235
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| Functional Studies |
FuncS: Use at an assay dependent dilution. Stimulates thymidine uptake by BaF3 cells expressing FGF receptors with an ED50 of <0.1 ng/ml corresponding to a specific activity of > 1 x 107 units/mg |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
-
Alternative names
Basic fibroblast growth factorBasic fibroblast growth factor bFGFBFGF
FGF 2FGF BFGF-2FGF2FGF2 basicFGF2_HUMANFGFBFibroblast growth factor 2Fibroblast growth factor 2 (basic)HBGF 2HBGF-2HBGF2HBGH 2HBGH2Heparin binding growth factor 2 precursorHeparin-binding growth factor 2Prostatropin
see all
FunctionPlays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
Tissue specificityExpressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
Sequence similaritiesBelongs to the heparin-binding growth factors family.
Post-translational
modificationsPhosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
Cellular localizationSecreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
References for FGF basic protein (ab50235)
ab50235
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
