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Anti-FGF1 antibody - Carboxyterminal end (ab76662)

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Overview

  • Product nameAnti-FGF1 antibody - Carboxyterminal endSee all FGF1 primary antibodies ...
  • Description
    Rabbit polyclonal to FGF1 - Carboxyterminal end
  • Tested applicationsIHC-P, WB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the C terminal region of human FGF1.

  • Positive controlMouse kidney tissue lysate and FGF1 transfected 293 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • Purification notesThis antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab76662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/10 - 1/50.
WB WB: 1/50 - 1/100. Predicted molecular weight: 17 kDa.Can be blocked with FGF1 peptide (ab154948).
ELISA ELISA: 1/1000.

Target

  • FunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
  • Sequence similaritiesBelongs to the heparin-binding growth factors family.
  • Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.

    • Target information above from: UniProt accession P05230 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Acidic fibroblast growth factor antibodyAFGF antibodyBeta endothelial cell growth factor antibody
        Beta-endothelial cell growth factor antibodyECGF antibodyECGF beta antibodyECGF-beta antibodyECGFA antibodyECGFB antibodyEndothelial Cell Growth Factor alpha antibodyEndothelial Cell Growth Factor beta antibodyFGF 1 antibodyFGF alpha antibodyFgf1 antibodyFGF1_HUMAN antibodyFGFA antibodyFibroblast Growth Factor 1 Acidic antibodyFibroblast growth factor 1 antibodyGLIO703 antibodyHBGF 1 antibodyHBGF-1 antibodyHBGF1 antibodyHeparin binding growth factor 1 antibodyHeparin binding growth factor 1 precursor antibodyHeparin-binding growth factor 1 antibody
      see all

    Anti-FGF1 antibody - Carboxyterminal end images

    • Western blot - FGF1 antibody - Carboxyterminal end (ab76662)
      Western blot - FGF1 antibody - Carboxyterminal end (ab76662)
      Anti-FGF1 antibody - Carboxyterminal end (ab76662) at 1/60 dilution + mouse kidney tissue lysate at 35 µg

      Predicted band size : 17 kDa
      Observed band size : 19 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
    • Western blot - FGF1 antibody - Carboxyterminal end (ab76662)
      Western blot - FGF1 antibody - Carboxyterminal end (ab76662)
      All lanes : Anti-FGF1 antibody - Carboxyterminal end (ab76662) at 1/60 dilution

      Lane 1 : Non-transfected 293 cell lysates
      Lane 2 : FGF1 transfected 293 cell lysates

      Lysates/proteins at 2 µg per lane.


      Predicted band size : 17 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-FGF1 antibody - Carboxyterminal end(ab76662)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-FGF1 antibody - Carboxyterminal end(ab76662)
      ab76662 at 1/50 dilution staining FGF1 Carboxyterminal in human Hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

    References for Anti-FGF1 antibody - Carboxyterminal end (ab76662)

    ab76662 has not yet been referenced specifically in any publications.

    Publishing research using ab76662 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab76662 Submit a Question for ab76662

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    I would like to purchase the immunogen peptide of anti-FGF1 antibody ab76662, could you please send me the catalouge reference number so that we can order the peptide?


    The blocking peptide for the anti-FGF1 antibody ab76662 has been added to our catalogue, the reference is ab154948 and the link to the datasheet is http://www.abcam.com/ab154948.

    I am wondering weather you have a blocking peptide for FGF1 antibody ab76662?
    Please let me know, thank you, best regards

    Thank you for contacting us.

    We contacted the source of the the anti-FGF1 antibody ab76662 and I can confirm that the immunogen peptide can be added to our catalogue. The price would €180.00 for 100µg.
    Could you please confirm that...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"