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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Overview
Product name
Anti-FGF1 antibody - N-terminal
See all FGF1 products (11) ...
Description
Rabbit polyclonal to FGF1 - N-terminal
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
KLH conjugated synthetic peptide selected from the N-terminal region of human FGF1.
Positive control
293 cell lysate transiently transfected with the FGF1 gene. Human colon carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
ab75928 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Growth factors >> FGF
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100.Predicted molecular weight: 17 kDa.
IHC-P: 1/10 - 1/50.
ELISA
ELISA: 1/1000ELISA: 1/1000
Target
Function
The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Sequence similarities
Belongs to the heparin-binding growth factors family.
Target information above from: UniProt accessionP05230
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Acidic fibroblast growth factor antibody
- AFGF antibody
- Beta endothelial cell growth factor antibody
see all
Database links
- Entrez Gene: 2246 Human
- Omim: 131220 Human
- SwissProt: P05230 Human
- Unigene: 483635 Human
Anti-FGF1 antibody - N-terminal images:
Western blot - FGF1 antibody - N-terminal (ab75928)
All lanes : Anti-FGF1 antibody - N-terminal (ab75928) at 1/50 dilution
Lane 1 : Non transfected 293T cell lysate
Lane 2 : 293T cell lysate transiently transfected with the FGF1 gene
Lysates/proteins at 12.5 µg per lane.
Predicted band size : 17 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF1 antibody - N-terminal (ab75928)
Immunohistochemical analysis of FGF1 in formalin-fixed, paraffin-embedded human colon carcinoma tissue using ab75928, at 1/10 dilution, followed by a peroxidase-conjugated secondary antibody and DAB staining.
References for Anti-FGF1 antibody - N-terminal (ab75928)
ab75928 has not yet been referenced specifically in any publications.
Publishing research using ab75928? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"