Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab54677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ELISA
  • Application notesELISA: Use at a dilution of 1/250 for 8 hrs.
    IHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
    • Sequence similaritiesBelongs to the heparin-binding growth factors family.
    • Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acidic fibroblast growth factor antibody
      • aFGF antibody
      • Beta endothelial cell growth factor antibody
      • Beta-endothelial cell growth factor antibody
      • ECGF antibody
      • ECGF beta antibody
      • ECGF-beta antibody
      • ECGFA antibody
      • ECGFB antibody
      • Endothelial Cell Growth Factor alpha antibody
      • Endothelial Cell Growth Factor beta antibody
      • FGF 1 antibody
      • FGF alpha antibody
      • Fgf1 antibody
      • FGF1_HUMAN antibody
      • FGFA antibody
      • Fibroblast Growth Factor 1 Acidic antibody
      • Fibroblast growth factor 1 antibody
      • GLIO703 antibody
      • HBGF 1 antibody
      • HBGF-1 antibody
      • HBGF1 antibody
      • Heparin binding growth factor 1 antibody
      • Heparin binding growth factor 1 precursor antibody
      • Heparin-binding growth factor 1 antibody
      see all

    Anti-FGF1 antibody images

    • FGF1 antibody (ab54677) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
    • Western blot against tagged recombinant protein immunogen using ab54677 FGF1 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
    • All lanes : Anti-FGF1 antibody (ab54677) at 1 µg/ml

      Lane 1 : Human kidney whole cell lysate (50µg)
      Lane 2 : Human liver whole cell lysate (50µg)
      Lane 3 : Human spleen whole cell lysate (50µg)
      Lane 4 : Human pancreas whole cell lysate (50µg)
      Lane 5 : Human colon whole cell lysate (50µg)
      Lane 6 : Human placenta whole cell lysate (50µg)
      Lane 7 : Human stomach whole cell lysate (50µg)
      Lane 8 : Mouse brain whole cell lysate (50µg)

      Secondary
      Goat anti-mouse antibody conjugated to HRP at 1/5000 dilution

    References for Anti-FGF1 antibody (ab54677)

    ab54677 has not yet been referenced specifically in any publications.

    Product Wall

    Application ELISA
    Sample Human Recombinant protein (human)
    Specification human
    Blocking step Milk as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Type Sandwich (Capture)
    Username

    Abcam user community

    Verified customer

    Submitted Sep 04 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"