Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab54677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ELISA
  • Application notesELISA: Use at a dilution of 1/250 for 8 hrs.
    IHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at a concentration of 1 - 5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.
    • Sequence similaritiesBelongs to the heparin-binding growth factors family.
    • Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
    • Information by UniProt
    • Database links
    • Alternative names
      • Acidic fibroblast growth factor antibody
      • aFGF antibody
      • Beta endothelial cell growth factor antibody
      • Beta-endothelial cell growth factor antibody
      • ECGF antibody
      • ECGF beta antibody
      • ECGF-beta antibody
      • ECGFA antibody
      • ECGFB antibody
      • Endothelial Cell Growth Factor alpha antibody
      • Endothelial Cell Growth Factor beta antibody
      • FGF 1 antibody
      • FGF alpha antibody
      • Fgf1 antibody
      • FGF1_HUMAN antibody
      • FGFA antibody
      • Fibroblast Growth Factor 1 Acidic antibody
      • Fibroblast growth factor 1 antibody
      • GLIO703 antibody
      • HBGF 1 antibody
      • HBGF-1 antibody
      • HBGF1 antibody
      • Heparin binding growth factor 1 antibody
      • Heparin binding growth factor 1 precursor antibody
      • Heparin-binding growth factor 1 antibody
      see all

    Anti-FGF1 antibody images

    • FGF1 antibody (ab54677) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
    • Western blot against tagged recombinant protein immunogen using ab54677 FGF1 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
    • All lanes : Anti-FGF1 antibody (ab54677) at 1 µg/ml

      Lane 1 : Human kidney whole cell lysate (50µg)
      Lane 2 : Human liver whole cell lysate (50µg)
      Lane 3 : Human spleen whole cell lysate (50µg)
      Lane 4 : Human pancreas whole cell lysate (50µg)
      Lane 5 : Human colon whole cell lysate (50µg)
      Lane 6 : Human placenta whole cell lysate (50µg)
      Lane 7 : Human stomach whole cell lysate (50µg)
      Lane 8 : Mouse brain whole cell lysate (50µg)

      Secondary
      Goat anti-mouse antibody conjugated to HRP at 1/5000 dilution

    References for Anti-FGF1 antibody (ab54677)

    ab54677 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ELISA
    Sample Human Recombinant protein (human)
    Specification human
    Blocking step Milk as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Type Sandwich (Capture)
    Username

    Abcam user community

    Verified customer

    Submitted Sep 04 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"