Anti-FGF21 antibody (ab137715)
Key features and details
- Rabbit polyclonal to FGF21
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FGF21 antibody
See all FGF21 primary antibodies -
Description
Rabbit polyclonal to FGF21 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 99-193 of the Human FGF21 protein (Q9NSA1).
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Positive control
- WB: 293T, A431, HeLa, HepG2, Molt-4, and Raji whole cell lysates. ICC/IF: HeLa cells. IHC-P: HS578T xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 22 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 22 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 26291 Human
- Omim: 609436 Human
- SwissProt: Q9NSA1 Human
- Unigene: 283015 Human
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Alternative names
- FGF 21 antibody
- FGF-21 antibody
- Fgf21 antibody
see all
Images
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All lanes : Anti-FGF21 antibody (ab137715) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
12% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa labelling FGF21 with ab137715 at 1/200 (panel 1) and co-stained with a DNA probe (panel 2).
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Immunohistochemical analysis of paraffin-embedded HS578T xenograft labelling FGF21 with ab137715 at 1/500.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab137715 has been referenced in 1 publication.
- Ferrer-Curriu G et al. The protective effect of fibroblast growth factor-21 in alcoholic cardiomyopathy: a role in protecting cardiac mitochondrial function. J Pathol 253:198-208 (2021). PubMed: 33125701