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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Overview
Product name
Anti-FGF21 antibody
See all FGF21 products (5) ...
Description
Rabbit polyclonal to FGF21
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide derived from within residues 1 - 100 of Human FGF21.
(Peptide available as ab66563.)
Positive control
This antibody gave a positive signal on Full length FGF-21 Recombinant Protein (Human)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Growth factors >> FGF
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
IHC-P: Use a concentration of 5 µg/ml
Application notes
Is unsuitable for or ICC/IF.
Target
Function
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
Sequence similarities
Belongs to the heparin-binding growth factors family.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ9NSA1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FGF 21 antibody
- FGF-21 antibody
- Fgf21 antibody
see all
Database links
- Entrez Gene: 26291 Human
- Entrez Gene: 56636 Mouse
- Entrez Gene: 170580 Rat
- Omim: 609436 Human
- SwissProt: Q9NSA1 Human
- SwissProt: Q9JJN1 Mouse
- SwissProt: Q8VI80 Rat
- Unigene: 283015 Human
see all
Anti-FGF21 antibody images:
Western blot - FGF21 antibody (ab66564)
Anti-FGF21 antibody (ab66564) at 1 µg/ml + FGF21 protein (ab54141) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 22 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
ab66564 recognizes the full length FGF-21 Human Recombinant Protein which has an expected molecular weight of ~19.5 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF21 antibody (ab66564)
IHC image of FGF21 staining in Human Liver Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66564, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-FGF21 antibody (ab66564)
ab66564 has not yet been referenced specifically in any publications.
Publishing research using ab66564? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"