Overview
- Product nameFGF4 Human ELISA KitSee all FGF4 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture extracts, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 50 pg/ml
- Range24.69 pg/ml - 18000 pg/ml
- Recovery
92 %
Sample specific recovery Sample type Average % Range Cell culture extracts 93.45 94% - 103% Tissue Extracts 91.96 82% - 102% - Species reactivityReacts with: Human
- Product overview
FGF (Fibroblast growth factors) constitutes a family of related 16-18 kDa proteins controlling normal growth and differentiation of mesenchymal, epithelial, and neuroectodermal cell types. FGF4 has potent transforming potential, apparently through an autocrine mechanism of action, and it is a potent angiogenic factor. It has also been extensively studied for its effects in embryogenesis.
The ab100517 Human FGF4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF4 cell lysate and tissue lysate. This assay employs an antibody specific for human FGF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human FGF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm
- Tested applicationsELISA more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 2X Cell Lysate Buffer Item J 1 x 5ml Assay Diluent 5x concentrated buffer. For Detection Antibody and HRP-Streptavidin concentrate diluent. 1 x 15ml Detection Antibody FGF-4: biotinylated anti-human FGF-4 2 vials FGF-4 Microplate (12 strips x 8 wells) coated with anti-human FGF-4 1 unit FGF-4 Standard Human (recombinant) 2 vials HRP-Streptavidin Concentrate 120x concentrated HRP-conjugated streptavidin 1 x 200µl Sample Diluent Buffer 5x concentrated buffer For Standard/Sample (cell lysate/tissue lysate) diluent. 1 x 10ml Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml -
Research Areas
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Alternative names
- Fibroblast growth factor 4HBGF4Heparin binding growth factor 4
- Heparin secretory transforming protein 1HSTHST1HSTF1KS3Transforming protein KS3
see all
- SwissProt: P08620 Human
Applications
Our Abpromise guarantee covers the use of ab100517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
FGF4 Human ELISA Kit images
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ELISA - FGF4 Human ELISA Kit (ab100517)
Protocols
References for FGF4 Human ELISA Kit (ab100517)
ab100517 has not yet been referenced specifically in any publications.
