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Product Name
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FGF4 antibody [19805.11]
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See all FGF4 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [19805.11] to FGF4
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
(species key)
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Hu
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Specificity
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Anti-Human FGF4 neutralizes the bioactivity of recombinant, human FGF4 but not that of bovine or human, recombinant FGF Basic, bovine or human, recombinant FGF Acidic, human, recombinant FGF 5, human, recombinant FGF6, or human, recombinant ECGF.
In indirect ELISA and immunoblotting, this antibody displays no cross-reactivity with bovine or human, recombinant FGF Basic, bovine or human, recombinant FGF Acidic, or human, recombinant ECGF.
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Tested applications
(see key)
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I-ELISA, Neut, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
I-ELISA: Use at a concentration of 0.5 µg/ml.
Neut: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.
Anti-Human FGF4 is tested for its ability to neutralize the bioactivity of rhFGF4 in a cell proliferation assay using FGF4 responsive NR6-3T3 fibroblasts. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhFGF4, which is present at five times its own EC50 (the concentration of rhFGF4 producing a one-half maximal bioactivity without antibody).
In this bioassay, rhFGF4 was pre-incubated with various dilutions of the antibody for 1 hour at 37C in a 96-well microtiter plate. Then antigen-antibody mixtures were added to quiescent confluent NR6-3T3 cultures in 0.1 ml containing 0.5 ng/ml rhFGF-4. This was incubated for 20 hours at 37C in a 5% CO humidified incubator and then pulsed for 2 hours with H-thymidine. Cells were harvested onto glass filters and the H-thymidine incorporation into DNA was measured.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factors
Cancer >> Growth factors >> FGF
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
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Relevance
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FGF4 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF4 signals through the FGFR 1c, 2c, 3c, and 4.
Fibroblast growth factor 4 is a member of the FGF family, a group of 7 homologous polypeptides that act upon a variety of cells and produce biological actions that may include cell proliferation, migration and differentiation. FGF4, also known as oncogene products hst1 and KFGF, has strong transforming potential and is a potent angiogenic factor, being expressed in several highly vascularized tumors.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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19805.11
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FGF4 antibody [19805.11] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for FGF4 antibody [19805.11] (ab10422)
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This product has been used in:
Wegehingel S et al. Rerouting of fibroblast growth factor 2 to the classical secretory pathway results in post-translational modifications that block binding to heparan sulfate proteoglycans. FEBS Lett 582:2387-92 (2008). PubMed: 18538671
If you publish research using ab10422 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to FGF4
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