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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Overview
Product name
Anti-FGF4 antibody - Carboxyterminal end
See all FGF4 products (4) ...
Description
Rabbit polyclonal to FGF4 - Carboxyterminal end
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminal of the human FGF4, identical to the related mouse sequence.
Positive control
Hela cell lysis and human lung cancer tissue sections.
Properties
Form
Lyophilised:
0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factors
Cancer >> Growth factors >> FGF
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
IHC-P: Use a concentration of 1 - 2 µg/ml.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Can transform NIH 3T3 cells from a human stomach tumor (hst) and from karposi's sarcoma (KS3). It has a mitogenic activity.
Sequence similarities
Belongs to the heparin-binding growth factors family.
Cellular localization
Secreted.
Target information above from: UniProt accessionP08620
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FGF-4 antibody
- Fgf4 antibody
- FGF4_HUMAN antibody
see all
Database links
- Entrez Gene: 2249 Human
- Entrez Gene: 14175 Mouse
- Entrez Gene: 116499 Rat
- Omim: 164980 Human
- SwissProt: P08620 Human
- SwissProt: P11403 Mouse
- Unigene: 1755 Human
- Unigene: 4956 Mouse
see all
Anti-FGF4 antibody - Carboxyterminal end images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF4 antibody (ab65974)
ab65974 at 1µg/ml staining FGF4 in human lung cancer tissue sections.
Western blot - FGF4 antibody (ab65974)
Anti-FGF4 antibody - Carboxyterminal end (ab65974) at 1 µg/ml + Hela cell lysis at 30 µg
Predicted band size : 22 kDa
Observed band size : 22 kDa
References for Anti-FGF4 antibody - Carboxyterminal end (ab65974)
This product has been referenced in:
- Dobrowolski Ret al. Loss of connexin43-mediated gap junctional coupling in the mesenchyme of limb buds leads to altered expression of morphogens in mice. Hum Mol Genet 18:2899-911 (2009).Read more (PubMed: 19439426) »
See 1 publication for this product
Publishing research using ab65974? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"