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Product Name 

FGF5 protein (Active)

Product type 

Proteins and Peptides

Peptide 

Recombinant Human FGF-5, a 27.6 kDa protein consisting of 252 amino acid residues.

Tested applications 
(see key)

FuncS, SDS-PAGE

Application notes 
(see key)

Recommended dilutions
FuncS: Use at an assay dependent dilution. The ED50, determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is < 0.5 ng/ml, corresponding to a specific activity of > 2 x 106 units/mg.

SDS-PAGE: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cancer >> Growth factors >> FGF
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF

 

Relevance 

FGF-5 is a secreted heparin binding growth factor belonging to the FGF family. Proteins of this family play a central role in prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-5 binds to FGFR 1c and 2c, and plays a regulatory role in the hair growth cycle.

 

Alternative names FGF5 protein (Active) (ab50229)

Purity 

>95% by SDS-PAGE

Purification notes 

Endotoxin level is less than 0.1 ng per µg (1EU/µg).

Form 

Lyophilised
Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2 to a concentration of 0.5–1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Repeated freeze thaw cycles will result in some loss of activity.

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Expression system 

E. coli

Sequence 

MAWAHGEKRL APKGQPGPAA TDRNPIGSSS RQSSSSAMSS SSASSSPAAS LGSQGSGLEQ SSFQWSPSGR RTGSLYCRVG IGFHLQIYPD GKVNGSHEAN MLSVLEIFAV SQGIVGIRGV FSNKFLAMSK KGKLHASAKF TDDCKFRERF QENSYNTYAS AIHRTEKTGR EWYVALNKRG KAKRGCSPRV KPQHISTHFL PRFKQSEQPE LSFTVTVPEK KNPPSPIKSK IPLSAPRKNT NSVKYRLKFR FG

 

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Search PubMed (MEDLINE) for references to FGF5

 

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