FGF7 Human ELISA Kit (ab100519)
- Product nameFGF7 Human ELISA KitSee all FGF7 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 0.5 pg/ml
- Range0.55 pg/ml - 400 pg/ml
> 93 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 93.24 81% - 98% Serum 112.9 102% - 123% Plasma 126.2 114% - 135%
- Species reactivityReacts with: Human
- Product overview
ab100519 FGF7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FGF7 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human FGF7 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human FGF7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent 5x concentrated buffer. For Standard/Sample (srum/ plasma samples/cell culture medium/urine) diluent. 1 x 15ml Detection Antibody FGF-7: biotinylated anti-human FGF-7 2 vials FGF 7 Standard Human (recombinant) 2 vials FGF-7 Microplate (12 strips x 8 wells) coated with anti-human FGF-7 1 unit Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- FGF 7Fibroblast growth factor 7HBGF 7
- Heparin binding growth factor 7Keratinocyte growth factorKGFOTTMUSP00000016341OTTMUSP00000016342RP23-386I21.3
Our Abpromise guarantee covers the use of ab100519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for FGF7 Human ELISA Kit (ab100519)
ab100519 has not yet been referenced specifically in any publications.