Anti-FGF8 antibody (ab81384)
Key features and details
- Rabbit polyclonal to FGF8
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FGF8 antibody
See all FGF8 primary antibodies -
Description
Rabbit polyclonal to FGF8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminal of Human FGF8.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells. -
Involvement in disease
Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6) [MIM:612702]. Kallmann syndrome is a disorder that associates hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some patients other developmental anomalies can be present, which include renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. In some cases anosmia may be absent or inconspicuous.
Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Developmental stage
In adults expression is restricted to the gonads. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2253 Human
- Entrez Gene: 14179 Mouse
- Entrez Gene: 29349 Rat
- Omim: 600483 Human
- SwissProt: P55075 Human
- SwissProt: P37237 Mouse
- Unigene: 57710 Human
- Unigene: 4012 Mouse
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Alternative names
- AIGF antibody
- Androgen induced growth factor antibody
- Androgen-induced growth factor antibody
see all
Images
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ab81384 staining FGF8 in Human Ovarian cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 37°C; antigen retrieval was by microwave heat mediation in a citrate buffer. Samples were incubated with primary antibody (2 μg/mL) for 2 hours at 37°C. A Biotin-conjugated secondary antibody was used.
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ab81384 at 1µg/ml staining FGF8 in Rat ovary tissue sections by Immunohistochemistry(Formalin/ PFA-fixed paraffin-embedded sections). The tissue underwent heat mediated antigen retrieval. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
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All lanes : Anti-FGF8 antibody (ab81384)
All lanes : Rat Ovary Tissue Lysate
Predicted band size: 27 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab81384 has been referenced in 6 publications.
- Hao Y et al. FGF8 induces epithelial-mesenchymal transition and promotes metastasis in oral squamous cell carcinoma. Int J Oral Sci 13:6 (2021). PubMed: 33649301
- Haid B et al. FGF8, FGF10 and FGF receptor 2 in foreskin of children with hypospadias: an analysis of immunohistochemical expression patterns and gene transcription. J Pediatr Urol 16:41.e1-41.e10 (2020). PubMed: 31718875
- Fex Svenningsen Å et al. Macrophage migration inhibitory factor (MIF) modulates trophic signaling through interaction with serine protease HTRA1. Cell Mol Life Sci 74:4561-4572 (2017). ICC ; Mouse . PubMed: 28726057
- Pei Y et al. FGF8 promotes cell proliferation and resistance to EGFR inhibitors via upregulation of EGFR in human hepatocellular carcinoma cells. Oncol Rep 38:2205-2210 (2017). PubMed: 28791365
- Hetzl AC et al. Fibroblast growth factor, estrogen, and prolactin receptor features in different grades of prostatic adenocarcinoma in elderly men. Microsc Res Tech 76:321-30 (2013). PubMed: 23362007
- Toh YC et al. Spatially organized in vitro models instruct asymmetric stem cell differentiation. Integr Biol (Camb) 3:1179-87 (2011). PubMed: 22028041