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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Overview
Product name
Anti-FGF9 antibody
See all FGF9 products (7) ...
Description
Rabbit polyclonal to FGF9
Tested applications
ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Chicken, Pig, Xenopus laevis
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human FGF9.
(Peptide available as ab86940.)
Positive control
This antibody gave a positive signal in the following lysates: Human Brain Tissue; Human Stomach Tumour Tissue; HEK293 Whole Cell; Mouse Brain Tissue; Mouse Kidney Tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 43 kDa (predicted molecular weight: 23 kDa).
Target
Function
May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
Tissue specificity
Glial cells.
Involvement in disease
Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness.
Sequence similarities
Belongs to the heparin-binding growth factors family.
Post-translational
modifications
Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
N-glycosylated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP31371
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FGF 9 antibody
- FGF-9 antibody
- FGF9 antibody
see all
Database links
- Entrez Gene: 378917 Chicken
- Entrez Gene: 2254 Human
- Entrez Gene: 14180 Mouse
- Entrez Gene: 396717 Pig
- Entrez Gene: 25444 Rat
- Entrez Gene: 378562 Xenopus laevis
- Omim: 600921 Human
- SwissProt: Q7ZZN4 Chicken
see all
Anti-FGF9 antibody images:
Immunocytochemistry/ Immunofluorescence - FGF9 antibody (ab71395)
ICC/IF image of ab71395 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71395, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, hek293 and MCF7 cells at 5µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF9 antibody (ab71395)
IHC image of FGF9 staining in FFPE human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71395, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - FGF9 antibody (ab71395)
All lanes : Anti-FGF9 antibody (ab71395) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Stomach (Human) Whole Cell Lysate - tumor tissue (ab29678)
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466) with FGF9 peptide (ab86940) at 1 µg/ml
Lane 5 : Stomach (Human) Whole Cell Lysate - tumor tissue (ab29678) with FGF9 peptide (ab86940) at 1 µg/ml
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with FGF9 peptide (ab86940) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 23 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
The peptide blocking data above shows that ab71395 detects a protein of approximately 50 kDa, thought to be FGF9. It is possible that this band is significantly higher than expected due to modifications at potential glycosylation sites.
Western blot - Anti-FGF9 antibody (ab71395)
All lanes : Anti-FGF9 antibody (ab71395) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Additional bands at : 105 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
References for Anti-FGF9 antibody (ab71395)
ab71395 has not yet been referenced specifically in any publications.
Publishing research using ab71395? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"