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ab9745
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab9743 for help.
There are no answered questions for ab9743
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - FGF9 antibody (ab9743)
All lanes : Anti-FGF9 antibody (ab9743) at 1/500 dilution
Lane 1 : Recombinant FGF9
Lane 2 : Rat cerebral cortex membrane whole tissue lysate. at 15 µg
Secondary
Mouse anti-rabbit IgG monoclonal at 1/1000 dilution
developed using the ECL technique
Exposure time : 30 seconds
Blocking Step: 1% Milk for 1 hour at 16°C
Gel Running Conditons: 15% gel Reduced, denaturing
Observed Bands: 23kDa recombinant FGF9 - 46kDa in cerebral cortex membrane fraction.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF9 antibody (ab9743)
ab9743 staining FGF9 in mouse lymph node tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye prior to blocking with 10% serum for 1 hour at 20ºC. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4ºC. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200.
This image is courtesy of an Anonymous Abreview.
FGF9 antibody for WB in Rat (9743)
FGF9 antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Mouse (9743)
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