FGF9 antibody (ab9743)

Product Name 

FGF9 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to FGF9

Immunogen 

Highly pure (>98%) recombinant mFGF-9 (mouse Fibroblast Growth Factor-9).

Reacts with 
(species key)

Ms

Tested applications 
(see key)

ELISA, Neut, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
Neutralization:To yield one-half maximal inhibition [ND₅₀] of the biological activity of mFGF-9 (1.5 ng/ml), a concentration of 0.025 - 0.06 µg/ml of this antibody is required.

ELISA:To detect mFGF-9 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mFGF-9.

Western Blot:To detect mFGF-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mFGF-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Cellular localization 

Secreted

Research areas 

Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF

Relevance 

FGF9 is a heparin binding growth factor, which is a member of the FGF family of proteins. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 is produced mainly by neurons and may have a role in glial cell growth and differentiation during development; gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Purity 

Immunogen affinity purified

Storage buffer 

PBS, pH 7.4, no preservative, sterile filtered

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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