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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Overview
Product name
Anti-FGF9 antibody
See all FGF9 products (7) ...
Description
Rabbit polyclonal to FGF9
Tested applications
IHC-P, WB, ELISA, Neutralisingmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Highly pure (>98%) recombinant mFGF-9 (mouse Fibroblast Growth Factor-9).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, pH 7.4, no preservative, sterile filtered
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
unknown
Light chain type
unknown
Research areas
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. (PubMed: 17192470)
WB: Use a concentration of 0.1 - 0.2 µg/ml.
ELISA: Use a concentration of 0.5 µg/ml(Allows the detection of 0.2 - 0.4 ng/well of recombinant mFGF-9 (using 100µl/well antibody solution).)
Neut: Use a concentration of 0.025 - 0.06 µg/ml.(for one-half maximal inhibition [ND50] of the biological activity of mFGF-9 (1.5 ng/ml).)
Target
Function
May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
Tissue specificity
Glial cells.
Involvement in disease
Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness.
Sequence similarities
Belongs to the heparin-binding growth factors family.
Post-translational
modifications
Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
N-glycosylated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP31371
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FGF 9 antibody
- FGF-9 antibody
- FGF9 antibody
see all
Database links
- Entrez Gene: 14180 Mouse
- Entrez Gene: 25444 Rat
- SwissProt: P54130 Mouse
- SwissProt: P36364 Rat
- Unigene: 8846 Mouse
- Unigene: 25174 Rat
Anti-FGF9 antibody images:
Western blot - FGF9 antibody (ab9743)
All lanes : Anti-FGF9 antibody (ab9743) at 1/500 dilution
Lane 1 : Recombinant FGF9
Lane 2 : Rat cerebral cortex membrane whole tissue lysate. at 15 µg
Secondary
Mouse anti-rabbit IgG monoclonal at 1/1000 dilution
developed using the ECL technique
Exposure time : 30 seconds
Blocking Step: 1% Milk for 1 hour at 16°C
Gel Running Conditons: 15% gel Reduced, denaturing
Observed Bands: 23kDa recombinant FGF9 - 46kDa in cerebral cortex membrane fraction.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGF9 antibody (ab9743)
ab9743 staining FGF9 in mouse lymph node tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200.
This image is courtesy of an Anonymous Abreview.
References for Anti-FGF9 antibody (ab9743)
This product has been referenced in:
- Arnaud-Dabernat Set al. FGFR3 contributes to intestinal crypt cell growth arrest. J Cell Physiol 216:261-8 (2008).Read more (PubMed: 18286540) »
- Arnaud-Dabernat Set al. FGFR3 is a negative regulator of the expansion of pancreatic epithelial cells. Diabetes 56:96-106 (2007). IHC-P; Mouse.Read more (PubMed: 17192470) »
See all 2 publications for this product
Publishing research using ab9743? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"