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Product Name
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FGF9 protein (Active)
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Product type
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Proteins and Peptides
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Peptide
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Recombinant full length FGF9 protein (Human), MW 23kDa. A 208 amino acid residue protein that contains a single, potential N-linked glycosylation site. Source: Insect cells.
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Specificity
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[Specific Activity: 6.6 x 105 units/mg]
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Tested applications
(see key)
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FuncS, SDS-PAGE
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Application notes
(see key)
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Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution.
Biological Activity: The activity was estimated by stimulation of 3H-thymidine incorporation in quiescent Balb/c3T3 cells. The ED50 for this effect is 1.5-2.0 ng/ml. Maximal effect for DNA synthesis was observed at 5-7 ng/ml rhFGF9.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
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Relevance
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FGF9 is a heparin binding growth factor, which is a member of the FGF family of proteins. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 is produced mainly by neurons and may have a role in glial cell growth and differentiation during development; gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: None
Buffer: None
Endotoxin level: < 0.1 ng per µg of FGF9.
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Purification notes
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Purity: > 95% by SDS-PAGE.
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Form
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Lyophilised
For reconstitution it is recommended to add sterile phosphate-buffered saline (or medium) containing at least 0.1% BSA/HSA (or FCS) to prepare a stock solution of no less than 100 µg/ml. For dilution it is recommended to use sterile phosphate-buffered saline containing at least 0.1% BSA/HSA or using medium with FCS for dilution.
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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