Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Overview
Product name
FGF9 protein
See all FGF9 products (5) ...
Protein description
Highly pure (>98%) recombinant mouse FGF9.
Expression host
E. coli
Properties
Purification notes
Sterile filtered Greater than 95% pure by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Form
Lyophilised:
Reconstitute using 50ul of sterile H20.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Additional notes
mFGF-9 is fully biologically active when compared to standards.
The ED50, as determined by the dose-dependent stimulation of thymidine uptake by BaF3 expressing FGF receptors, is less than 0.5 ng/ml, corresponding to a specific activity of >2 X 106 units/mg.
Murine FGF-9 exerts full activity on both murine and human cells.
For most in vitro applications, FGF-9 exerts its biological activity in the concentration range of 0.1 to 20.0 ng/ml.
Sequence notes
Recombinan t Murine FGF-9 is a 23.3 kDa protein containing 205 amino acid residues: PLGEVGS YFG VQDAVPFGN V PVLPVDSP VL LNDHLGQ SEA GGLPRG PAVT DLDHL KGILRRRQLY CRTGF HLEI FPNGTI QGT RKDHSRF GI LEFISIAV G LVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFE E NWYNTYSS NL YKHVDTG RRY YVALNK DGTP REGTR TKRHQKFTHF LPRPV DPDK VPELYK DIL SQS
Research areas
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Application notes
This peptide can be used with studies using ab9743.
Protein info
Function
May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
Tissue specificity
Glial cells.
Involvement in disease
Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness.
Sequence similarities
Belongs to the heparin-binding growth factors family.
Post-translational
modifications
Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
N-glycosylated.
Cellular localization
Secreted.
Target information above from: UniProt accessionP31371
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for FGF9 protein (ab9745)
ab9745 has not yet been referenced specifically in any publications.
Publishing research using ab9745? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"