Overview

  • Product nameAnti-FGFR1 Oncogene Partner antibodySee all FGFR1 Oncogene Partner primary antibodies ...
  • Description
    Rabbit polyclonal to FGFR1 Oncogene Partner
  • Specificityab53071 detects endogenous levels of total FGFR1 protein.
  • Tested applicationsWB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide derived from human FGFR1.

  • Positive control
    • Human breast carcinoma tissue and HepG2 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab53071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ELISA
  • Application notesELISA: 1/10000.
    IHC-P: 1/50 - 1/100. Antigen retrieval method: sodium citrate buffer (pH 6.0)
    WB: 1/500 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for anchoring microtubules to the centrosomes.
    • Tissue specificityUbiquitous. Highly expressed in heart, liver, muscle, kidney, intestine, colon, adrenal gland, prostate, testis, and pancreas.
    • Involvement in diseaseNote=A chromosomal aberration involving FGFR1OP may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
    • Sequence similaritiesBelongs to the FGFR1OP family.
      Contains 1 LisH domain.
    • Cellular localizationCytoplasm > cytoskeleton > centrosome. Associated with gamma-tubulin.
    • Information by UniProt
    • Database links
    • Alternative names
      • FGFR1 oncogene partner antibody
      • FGFR1OP antibody
      • Fibroblast growth factor receptor 1 oncogene partner antibody
      • FOP antibody
      • FR1OP_HUMAN antibody
      • OTTHUMP00000017612 antibody
      • OTTHUMP00000017613 antibody
      see all

    Anti-FGFR1 Oncogene Partner antibody images

    • All lanes : Anti-FGFR1 Oncogene Partner antibody (ab53071) at 1/500 dilution

      Lane 1 : HepG2 cell extract
      Lane 2 : HepG2 cell extract with immunising peptide


      Predicted band size : 92 kDa
      Observed band size : 92 kDa
    • ab53071 at 1/50 dilution staining FGFR1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

    References for Anti-FGFR1 Oncogene Partner antibody (ab53071)

    This product has been referenced in:
    • Tarkkonen KM  et al. Differential roles of fibroblast growth factor receptors (FGFR) 1, 2 and 3 in the regulation of S115 breast cancer cell growth. PLoS One 7:e49970 (2012). WB . Read more (PubMed: 23185502) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Pig Tissue lysate - whole (Fetal Heart)
    Loading amount 50 µg
    Specification Fetal Heart
    Gel Running Conditions Reduced Denaturing (4-12% invitrogen mini gel)
    Blocking step Western Breeze blocking (Invitrogen) as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Dr. Vanessa Hall

    Verified customer

    Submitted Nov 05 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"