Overview

  • Product nameAnti-FGFR1 Oncogene Partner antibody
    See all FGFR1 Oncogene Partner primary antibodies
  • Description
    Rabbit polyclonal to FGFR1 Oncogene Partner
  • Specificityab53071 detects endogenous levels of total FGFR1 protein.
  • Tested applicationsWB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide derived from human FGFR1.

  • Positive control
    • Human breast carcinoma tissue and HepG2 cell extracts.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab53071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ELISA
  • Application notesELISA: 1/10000.
    IHC-P: 1/50 - 1/100. Antigen retrieval method: sodium citrate buffer (pH 6.0)
    WB: 1/500 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRequired for anchoring microtubules to the centrosomes.
    • Tissue specificityUbiquitous. Highly expressed in heart, liver, muscle, kidney, intestine, colon, adrenal gland, prostate, testis, and pancreas.
    • Involvement in diseaseNote=A chromosomal aberration involving FGFR1OP may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
    • Sequence similaritiesBelongs to the FGFR1OP family.
      Contains 1 LisH domain.
    • Cellular localizationCytoplasm > cytoskeleton > centrosome. Associated with gamma-tubulin.
    • Information by UniProt
    • Database links
    • Alternative names
      • FGFR1 oncogene partner antibody
      • FGFR1OP antibody
      • Fibroblast growth factor receptor 1 oncogene partner antibody
      • FOP antibody
      • FR1OP_HUMAN antibody
      • OTTHUMP00000017612 antibody
      • OTTHUMP00000017613 antibody
      see all

    Anti-FGFR1 Oncogene Partner antibody images

    • All lanes : Anti-FGFR1 Oncogene Partner antibody (ab53071) at 1/500 dilution

      Lane 1 : HepG2 cell extract
      Lane 2 : HepG2 cell extract with immunising peptide


      Predicted band size : 92 kDa
      Observed band size : 92 kDa
    • ab53071 at 1/50 dilution staining FGFR1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.

    References for Anti-FGFR1 Oncogene Partner antibody (ab53071)

    This product has been referenced in:
    • Tarkkonen KM  et al. Differential roles of fibroblast growth factor receptors (FGFR) 1, 2 and 3 in the regulation of S115 breast cancer cell growth. PLoS One 7:e49970 (2012). WB . Read more (PubMed: 23185502) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Pig Tissue lysate - whole (Fetal Heart)
    Loading amount 50 µg
    Specification Fetal Heart
    Gel Running Conditions Reduced Denaturing (4-12% invitrogen mini gel)
    Blocking step Western Breeze blocking (Invitrogen) as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Dr. Vanessa Hall

    Verified customer

    Submitted Nov 05 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"