Anti-FGFR1 (phospho Y653 + Y654) antibody (ab111124)

Overview

• Product nameAnti-FGFR1 (phospho Y653 + Y654) antibodySee all FGFR1 primary antibodies ...
• Description
  Mouse monoclonal to FGFR1 (phospho Y653 + Y654)
• Specificityab111124 detects overexpressed levels of Tyr653/654 phosphorylated FGFR1. Slight cross-reactivity observed with activated PDGF and insulin/IGF-I receptors. Does not cross-react with other tyrosine-phosphorylated proteins. Predicted to react with FGFR2, FGFR3 and FGFR4 due to sequence homology.
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  A synthetic phosphopeptide conjugated to KLH, corresponding to residues surrounding Tyr653/654 of Human FGFR1.

• Positive controlHuman pancreas tissue.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.50
  Constituents: 0.24% HEPES, 0.88% Sodium chloride, BSA, 50% Glycerol
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• IsotypeIgG2b
• Research Areas
  • Cancer
  • Growth factors
  • FGF

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Signal Transduction
  • Growth Factors/Hormones
  • FGF

  • Cardiovascular
  • Angiogenesis
  • Growth Factors
  • FGF
  • FGF Receptors

  • Neuroscience
  • Neurology process
  • Neurogenesis

Applications

Target

• FunctionReceptor for basic fibroblast growth factor. Receptor for FGF23 in the presence of KL (By similarity). A shorter form of the receptor could be a receptor for FGF1 (aFGF).
• Tissue specificityDetected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
• Involvement in diseaseDefects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
  Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
  Defects in FGFR1 are the cause of Kallmann syndrome type 2 (KAL2) [MIM:147950]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some cases, midline cranial anomalies (cleft lip/palate and imperfect fusion) are present and anosmia may be absent or inconspicuous.
  Defects in FGFR1 are the cause of osteoglophonic dysplasia (OGD) [MIM:166250]; also known as osteoglophonic dwarfism. OGD is characterized by craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as by rhizomelic dwarfism and nonossifying bone lesions. Inheritance is autosomal dominant.
  Defects in FGFR1 are the cause of trigonocephaly non-syndromic (TRICEPH) [MIM:190440]; also known as metopic craniosynostosis. The term trigonocephaly describes the typical keel-shaped deformation of the forehead resulting from premature fusion of the frontal suture. Trigonocephaly may occur also as a part of a syndrome.
  Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with ZMYM2. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow.
  Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1OP. Insertion ins(12;8)(p11;p11p22) with FGFR1OP2. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP2-FGFR1, FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
  Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(8;9)(p12;q33) with CEP110. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein CEP110-FGFR1 is found in the cytoplasm, exhibits constitutive kinase activity and may be responsible for the transforming activity.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
  Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsBinding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.
• Cellular localizationMembrane. Nucleus. Cytoplasm. Cytoplasmic vesicle

Target information above from: UniProt accession P11362 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2260 Human
  • Omim: 136350 Human
  • SwissProt: P11362 Human
  • Unigene: 264887 Human

• Alternative names
  Basic fibroblast growth factor receptor 1 antibodybFGF-R-1 antibodyBFGFR antibody

  CD331 antibodyCEK antibodyFGFBR antibodyFGFR 1 antibodyFGFR-1 antibodyFGFR1 antibodyFGFR1_HUMAN antibodyFLG antibodyFLT-2 antibodyFLT2 antibodyFms-like tyrosine kinase 2 antibodyHBGFR antibodyKAL2 antibodyProto-oncogene c-Fgr antibody

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Anti-FGFR1 (phospho Y653 + Y654) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGFR1 (phospho Y653 + Y654) antibody (ab111124)
  ab111124, at 1/50 dilution, staining FGFR1 in formalin-fixed, paraffin-embedded Human Pancreas tissue by Immunohistochemistry followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.