Anti-FGFR1 (phospho Y766) antibody (ab59180)

Overview

  • Product nameAnti-FGFR1 (phospho Y766) antibodySee all FGFR1 primary antibodies ...
  • Description
    Rabbit polyclonal to FGFR1 (phospho Y766)
  • SpecificityDetects endogenous levels of FGFR1 only when phosphorylated at tyrosine 766.
  • Tested applicationsIHC-P, ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide from around the phosphorylation site of tyrosine 766 (QEYPLD) of human FGFR1

  • Positive control
    • Human breast carcinoma tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH.

Properties

Applications

Our Abpromise guarantee covers the use of ab59180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
ELISA 1/5000.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000.

Target

  • FunctionReceptor for basic fibroblast growth factor. Receptor for FGF23 in the presence of KL (By similarity). A shorter form of the receptor could be a receptor for FGF1 (aFGF).
  • Tissue specificityDetected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
  • Involvement in diseaseDefects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
    Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
    Defects in FGFR1 are the cause of Kallmann syndrome type 2 (KAL2) [MIM:147950]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some cases, midline cranial anomalies (cleft lip/palate and imperfect fusion) are present and anosmia may be absent or inconspicuous.
    Defects in FGFR1 are the cause of osteoglophonic dysplasia (OGD) [MIM:166250]; also known as osteoglophonic dwarfism. OGD is characterized by craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as by rhizomelic dwarfism and nonossifying bone lesions. Inheritance is autosomal dominant.
    Defects in FGFR1 are the cause of trigonocephaly non-syndromic (TRICEPH) [MIM:190440]; also known as metopic craniosynostosis. The term trigonocephaly describes the typical keel-shaped deformation of the forehead resulting from premature fusion of the frontal suture. Trigonocephaly may occur also as a part of a syndrome.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with ZMYM2. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1OP. Insertion ins(12;8)(p11;p11p22) with FGFR1OP2. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP2-FGFR1, FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(8;9)(p12;q33) with CEP110. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein CEP110-FGFR1 is found in the cytoplasm, exhibits constitutive kinase activity and may be responsible for the transforming activity.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.
  • Cellular localizationMembrane. Nucleus. Cytoplasm. Cytoplasmic vesicle
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic fibroblast growth factor receptor 1 antibody
    • bFGF R antibody
    • bFGF-R-1 antibody
    • BFGFR antibody
    • C FGR antibody
    • CD 331 antibody
    • CD331 antibody
    • CD331 antigen antibody
    • CEK antibody
    • FGFBR antibody
    • FGFR 1 antibody
    • FGFR-1 antibody
    • FGFR1 antibody
    • FGFR1_HUMAN antibody
    • Fibroblast growth factor receptor 1 antibody
    • FLG antibody
    • FLG protein antibody
    • FLJ14326 antibody
    • FLT 2 antibody
    • FLT-2 antibody
    • FLT2 antibody
    • FMS like tyrosine kinase 2 antibody
    • Fms related tyrosine kinase 2 antibody
    • Fms related tyrosine kinase 2 Pfeiffer syndrome antibody
    • Fms-like tyrosine kinase 2 antibody
    • H2 antibody
    • H3 antibody
    • H4 antibody
    • H5 antibody
    • HBGFR antibody
    • Heparin binding growth factor receptor antibody
    • HH2 antibody
    • HRTFDS antibody
    • Hydroxyaryl protein kinase antibody
    • KAL 2 antibody
    • KAL2 antibody
    • MFR antibody
    • N SAM antibody
    • N sam tyrosine kinase antibody
    • N-SAM antibody
    • OGD antibody
    • Protein tyrosine kinase antibody
    • Proto-oncogene c-Fgr antibody
    • Tyrosylprotein kinase antibody
    see all

Anti-FGFR1 (phospho Y766) antibody images

  • All lanes : Anti-FGFR1 (phospho Y766) antibody (ab59180)

    Lane 1 : EGF-treated HepG2 cell extract
    Lane 2 : EGF-treated HepG2 cell extract with blocking phosphopeptide
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab59180 with and without the addition of the synthetic phosphopeptide derived from human FGFR1 around the phosphorylation site of tyrosine 766.
  • ab59180 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab59180 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-FGFR1 (phospho Y766) antibody (ab59180)

This product has been referenced in:
  • Zou L  et al. Fibronectin induces endothelial cell migration through ß1 integrin and Src-dependent phosphorylation of fibroblast growth factor receptor-1 at tyrosines 653/654 and 766. J Biol Chem 287:7190-202 (2012). WB ; Human, Mouse . Read more (PubMed: 22247553) »

See 1 Publication for this product

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Thank you for contacting us.

I have conducted a search through our catalogue for antibodies against pFGFR1 and pFGFR4 which do no cross-react with the other FGFRs.

Ab59180 is specific against FGFR1 when it is phosphorilated at Tyr76...

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Thank you for your reply. I am sending a free of charge vial of ab109731 on the order *** which should arrive shortly. Please let me know if you have any questions or if there is anything else that we can do for you.

Thank you for submitting your recent review of ab59180. I am sorry to see that the results were so poor with this antibody. I do have a couple of suggestions that might help improve the results, however we do fully guarantee our products in tested spec...

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Application Western blot
Sample Human Cell lysate - whole cell (U87 (Glioblastoma))
Loading amount 50 µg
Specification U87 (Glioblastoma)
Treatment siRNA FGFR1
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Nov 30 2011

Thank you for contacting us. Indeed, crossreactivity is always a very important information for an antibody. Sequence alignment and experimental data can help to predict/determine this. We receive these antibodies from a collaborating l...

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