Anti-FGFR1 (phospho Y766) antibody (ab59180)

Overview

• Product nameAnti-FGFR1 (phospho Y766) antibodySee all FGFR1 primary antibodies ...
• Description
  Rabbit polyclonal to FGFR1 (phospho Y766)
• SpecificityDetects endogenous levels of FGFR1 only when phosphorylated at tyrosine 766.
• Tested applicationsIHC-P, ELISA, ICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic phosphopeptide from around the phosphorylation site of tyrosine 766 (QEY^PLD) of human FGFR1

• Positive controlHuman breast carcinoma tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Growth factors
  • FGF

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Signal Transduction
  • Growth Factors/Hormones
  • FGF

  • Cardiovascular
  • Angiogenesis
  • Growth Factors
  • FGF
  • FGF Receptors

  • Neuroscience
  • Neurology process
  • Neurogenesis

Applications

Target

• FunctionReceptor for basic fibroblast growth factor. Receptor for FGF23 in the presence of KL (By similarity). A shorter form of the receptor could be a receptor for FGF1 (aFGF).
• Tissue specificityDetected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
• Involvement in diseaseDefects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
  Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
  Defects in FGFR1 are the cause of Kallmann syndrome type 2 (KAL2) [MIM:147950]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some cases, midline cranial anomalies (cleft lip/palate and imperfect fusion) are present and anosmia may be absent or inconspicuous.
  Defects in FGFR1 are the cause of osteoglophonic dysplasia (OGD) [MIM:166250]; also known as osteoglophonic dwarfism. OGD is characterized by craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as by rhizomelic dwarfism and nonossifying bone lesions. Inheritance is autosomal dominant.
  Defects in FGFR1 are the cause of trigonocephaly non-syndromic (TRICEPH) [MIM:190440]; also known as metopic craniosynostosis. The term trigonocephaly describes the typical keel-shaped deformation of the forehead resulting from premature fusion of the frontal suture. Trigonocephaly may occur also as a part of a syndrome.
  Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with ZMYM2. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow.
  Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1OP. Insertion ins(12;8)(p11;p11p22) with FGFR1OP2. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP2-FGFR1, FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
  Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(8;9)(p12;q33) with CEP110. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein CEP110-FGFR1 is found in the cytoplasm, exhibits constitutive kinase activity and may be responsible for the transforming activity.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
  Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsBinding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.
• Cellular localizationMembrane. Nucleus. Cytoplasm. Cytoplasmic vesicle

Target information above from: UniProt accession P11362 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2260 Human
  • Entrez Gene: 14182 Mouse
  • Entrez Gene: 79114 Rat
  • Omim: 136350 Human
  • SwissProt: P11362 Human
  • SwissProt: P16092 Mouse
  • SwissProt: Q04589 Rat
  • Unigene: 264887 Human

  • Unigene: 265716 Mouse
  • Unigene: 207203 Rat
  • Unigene: 9797 Rat

  see all

• Alternative names
  Basic fibroblast growth factor receptor 1 antibodybFGF R antibodybFGF-R-1 antibody

  BFGFR antibodyC FGR antibodyCD 331 antibodyCD331 antibodyCD331 antigen antibodyCEK antibodyFGFBR antibodyFGFR 1 antibodyFGFR-1 antibodyFGFR1 antibodyFGFR1_HUMAN antibodyFibroblast growth factor receptor 1 antibodyFLG antibodyFLG protein antibodyFLJ14326 antibodyFLT 2 antibodyFLT-2 antibodyFLT2 antibodyFMS like tyrosine kinase 2 antibodyFms related tyrosine kinase 2 antibodyFms related tyrosine kinase 2 Pfeiffer syndrome antibodyFms-like tyrosine kinase 2 antibodyH2 antibodyH3 antibodyH4 antibodyH5 antibodyHBGFR antibodyHeparin binding growth factor receptor antibodyHydroxyaryl protein kinase antibodyKAL 2 antibodyKAL2 antibodyMFR antibodyN SAM antibodyN sam tyrosine kinase antibodyProtein tyrosine kinase antibodyProto-oncogene c-Fgr antibodyTyrosylprotein kinase antibody

  see all

Anti-FGFR1 (phospho Y766) antibody images

• 

  Western blot - FGFR1 (phospho Y766) antibody (ab59180)
  All lanes : Anti-FGFR1 (phospho Y766) antibody (ab59180)
  
  Lane 1 : EGF-treated HepG2 cell extract
  Lane 2 : EGF-treated HepG2 cell extract with blocking phosphopeptide
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FGFR1 (phospho Y766) antibody (ab59180)
  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab59180 with and without the addition of the synthetic phosphopeptide derived from human FGFR1 around the phosphorylation site of tyrosine 766.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 (phospho Y766) antibody (ab59180)

  ab59180 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab59180 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.