FGFR3 antibody (ab10649)

Product Name 

FGFR3 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to FGFR3

Immunogen 

Synthetic peptide: KDLLPPAPPSSGGSRT, corresponding to amino acids 792-806 of the cytoplasmic region of Human FGFR3 with N-terminal added lysine. The peptide is conjugated to KLH with glutaraldehyde.
BLAST 'K-DLLPPAPPSSGGSRT' with or

Reacts with 
(species key)

Hu

Specificity 

Does not react with FGFR1 or FGFR2.

Tested applications 
(see key)

IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-P: 1/1 - 1/250.

IP: 1/2000 as determined by immunoprecipitation using a whole cell lysate of transfected 293T cells expressing recombinant human FGFR3.

WB: 1/1000 as determined by western blotting using a whole cell extract of transfected 293T cells expressing recombinant human FGFR3. Detects a band of approximately 110-120 kDa.

The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin embedding.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole cell lysates of transfected 293T (embryonic kidney) cells expressing recombinant human FGFR3.

Cellular localization 

Single-pass type I membrane protein

Research areas 

Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Cancer >> Growth factors >> FGF
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF Receptors

Relevance 

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22-week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle. Epithelial cells show exclusively isoform 2 transcripts while fibroblastic cells show a mixture of isoform 1 and isoform 2.


Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation in a wide variety of cells of mesodermal, ectodermal and endodermal origin. FGFs are substantially involved in normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. They have also been implicated in pathological conditions such as tumorigenesis and metastasis. The FGF family consists of at least seventeen members designated FGF1 through FGF17. To date, four genes encoding for high affinity cell surface FGF receptors (FGFRs) have been identified: FGFR1 [flg1, cek1], FGFR2 [bek, cek3], FGFR3 [cek2] and FGFR4.

Soluble, secreted or possibly cleaved forms of FGFR1 and FGFR2 have also been found in body fluids or were artificially constructed. FGFRs are members of the tyrosine kinase family of growth factor receptors. They are glycosylated 110-150 kD proteins that are constructed of an extracellular ligand binding region with either two or typically three immunoglobulin (Ig)-like domains and an eight amino acid ‘acidic box’, a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3. FGFRs exhibit overlapping recognition and redundant specificity. One receptor type may bind several of the FGFs with a similar affinity. Also one FGF type may bind similarly to several distinct receptors. This accounts for the rather identical effects of different FGF ligands on common cell types. FGFs binding to cellular FGFRs depends on, or is markedly facilitated by, the low-affinity interaction of FGFs with the polysaccharide component of cell surface or extracellular matrix heparan sulfate proteoglycans (HSPG). For example, perlecan, a basement membrane HSPG, promotes high affinity binding of FGF2 in vitro and angiogenesis in vivo. Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ and are dictated by the intracellular domain.

FGFR3 is widely expressed in many fetal and adult human and animal tissues. FGFR3 expression profile largely correlates with its tissue specific expression at the mRNA level. It is considered the only FGFR expressed in the Organ of Corti of the rat cochlea. Tissue cultured cells transfected with the full length FGFR3 cDNA display the expected membrane localization of the receptor. Interestingly, nuclear localization (nucleoli excluded) of FGFR3 attributable to a 110kD splice variant, has been reported for normal and breast cancer cells. Deletions of chromosome 4p encompassing the FGFR3 gene cause the Wolf-Hirshhorn syndrome (growth failure, mental retardation, cardiac and bone malformations). Achondroplasia is an inherited disorder in which growth abnormality of bone or cartilage leads to skeletal maldevelopment and dwarfism. It is associated with recurrent mutations of a single amino acid in the transmembrane domain of the FGFR3 protein.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Conjugation notes 

The peptide immunogen is conjugates to KLH with glutaraldehyde.

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

The product is affinity purified on an immunizing peptide-agarose column.

Form 

Liquid

Concentration 

Batch dependent within range: 1.40 - 1.40 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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