FGFR3 antibody (ab10651)

Product Name 

FGFR3 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to FGFR3

Immunogen 

Synthetic peptide: AEEELVEADEAGSVK conjugated with glutaraldehyde to KLH, corresponding to amino acids 359-372 of Human FGFR3 with a C-terminal.
BLAST 'AEEELVEADEAGSVK' with or

Reacts with 
(species key)

Hu

Specificity 

No reaction with human FGFR1 and FGFR2 is detected.

Tested applications 
(see key)

IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/1000 (determined by indirect immunoperoxidase staining of protease-digested, formalin-fixed, paraffin-embedded human and animal tissue sections).

IP: 1/500.(determined by immunoprecipitation of FGFR3 using a whole cell lysate of 293T cells transfected with the human FGFR3 full length gene).

WB: 1/500. Detects a band of approximately 110 and 120 kDa. (determined by western blotting using an extract of 293T cells transfected with the human FGFR-3 full length gene).

The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole cell lysate of 293T cells transfected with the FGFR3 full length gene.

Cellular localization 

Single-pass type I membrane protein

Research areas 

Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Cancer >> Growth factors >> FGF
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF Receptors

Relevance 

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22-week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle. Epithelial cells show exclusively isoform 2 transcripts while fibroblastic cells show a mixture of isoform 1 and isoform 2.


Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (MW 17-38 kD) that are potent physiological regulators of growth and differentiation for a wide variety of cells of mesodermal, ectodermal and endodermal origin. FGFs are substantially involved in normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, and in hematopoiesis as well as in tissue remodelling and maintenance. They have also been implicated in pathological conditions such as tumorigenesis and metastasis. The FGF family consists of at least seventeen members designated FGF1 through FGF17. To date, four genes encoding for high affinity cell surface FGF receptors (FGFRs) have been identified: FGFR1 [flg-1(fms-like gene 1)]; FGFR2 [bek (bacterial expressed kinase gene product)]; FGFR3 (cek-2) and by alternative splicing have been reported. Soluble, secreted or possibly cleaved forms of FGFR-1 and FGFR-2 have also been found in body fluids or were artificially constructed. An example is a soluble FGF-binding protein containing the extracellular region of FGFR3 and the secreted form of placental alkaline phosphatase (FRAP3).

FGFRs are members of the tyrosine kinase family of growth factor receptors. They are glycosylated 110-150 kD proteins consiting of an extracellular domain, a single transmembrane region and a cytoplasmic split tyrosine kinase domain, which is activated following ligand binding and receptor dimerization. The extracellular, ligand binding, region is constructed with either two (beta type) or typically three (alpha-type) immunoglobulin (Ig)-like domains, and an eight amino acid ‘acidic box’. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3. FGFRs exhibit overlapping recognition and redundant specificity. One receptor type may bind several of the FGFs with a similar affinity. Also, one FGF type may bind similarly to several distinct receptors. This accounts for the rather identical effects of different FGF ligands on common cell types. FGFs binding to cellular FGFRs depend on, or is markedly facilitated by the low-affinity interaction of FGFs with the polysaccharide component of cell surface or extracellular matrix heparan sulfate proteoglycans (HSPG). Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signalling molecules leads to DNA synthesis or differentiation. The signalling and biological responses elicited by distinct FGFRs substantially differ and are dictated by the intracellular domain. FGFR3 is widely expressed in many fetal and adult human and animal tissues. The FGFR3 expression profile largely correlates with its tissue specific expression at the mRNA level.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 10mM Sodium Phosphate

Material safety datasheets (MSDS) for this product:
Sodium Azide MSDS
Sodium Phosphate MSDS

Purification notes 

The product is affinity purified on an immunizing peptide-agarose column.

Form 

Liquid

Concentration 

Batch dependent within range: 1.20 - 1.20 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for FGFR3 antibody (ab10651)

  This product has been used in: (two most recent references)

Page RL et al. Induction of stem cell gene expression in adult human fibroblasts without transgenes. Cloning Stem Cells 11:417-26 (2009). ICC/IF; Human. PubMed: 19622035

Terry J et al. Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin prevents synovial sarcoma proliferation via apoptosis in in vitro models. Clin Cancer Res 11:5631-8 (2005). PubMed: 16061882
If you publish research using ab10651 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding FGFR3 antibody

Customer FAQs regarding FGFR3 antibody

Protocols for FGFR3 antibody

Price and availability of products related to FGFR3 antibody

FGFR3 antibody - related products: