|
|
|
|
Western blot - FGFR3 antibody (ab52247)
(enlarge)
|
|
|
|
|
Product Name
|
FGFR3 antibody
|
| |
See all FGFR3 antibodies (6)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to FGFR3
|
|
Immunogen
|
Synthetic peptide derived from the extracellular region of human FGFR3.
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
ab52247 endogenous levels of total FGFR3 protein.
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/10000.
WB: 1/300 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
LOVO cells.
|
|
Cellular localization
|
Single-pass type I membrane protein
|
|
Research areas
|
Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Cancer >> Growth factors >> FGF
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF Receptors
|
|
Relevance
|
The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22-week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle. Epithelial cells show exclusively isoform 2 transcripts while fibroblastic cells show a mixture of isoform 1 and isoform 2.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at -20°C. Stable for 12 months at -20°C
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this FGFR3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for FGFR3 antibody images, references, products related to ab52247 and other tools.
|
| |
FGFR3 antibody images:
|
 Western blot - FGFR3 antibody (ab52247)
All lanes : FGFR3 antibody (ab52247) at 1/300 dilution
Lane 1 : LOVO cell extract; without peptide
Lane 2 : LOVO cell extract; with peptide
Predicted band size : 88 kDa
Observed band size : 88 kDa
|
| |
| |
|
Search PubMed (MEDLINE) for references to FGFR3
|
| |
FGFR3 antibody - more information
|
Customer reviews (feedback) regarding FGFR3 antibody |
Customer FAQs regarding FGFR3 antibody |
Protocols for FGFR3 antibody |
Price and availability of products related to FGFR3 antibody |
| |
FGFR3 antibody - related products:
|
|
|
| |
FGFR3 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about FGFR3 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
