FGFR3 protein (Active) (ab60857)

Product Name 

FGFR3 protein (Active)

Product type 

Proteins and Peptides

Peptide 

Recombinant fragment, corresponding to amino acids 397-end of Human FGFR3, was expressed by baculovirus in Sf9 insect cells using an N-terminal tag, MW 73kDa.

Tested applications 
(see key)

FuncS, SDS-PAGE

Application notes 
(see key)

Recommended dilutions
FuncS: Kinase assay: Use at an assay dependent dilution.

Specific Activity: 112 nmol/min/mg.


SDS-PAGE: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Stem Cells >> Neural Stem Cells >> Glial Restricted Lineage >> Astrocyte
Cancer >> Growth factors >> FGF
Signal Transduction >> Growth Factors/Hormones >> FGF
Cardiovascular >> Angiogenesis >> Growth Factors >> FGF >> FGF Receptors

Relevance 

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22-week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle. Epithelial cells show exclusively isoform 2 transcripts while fibroblastic cells show a mixture of isoform 1 and isoform 2.

Storage buffer 

Preservative: None
Constituents: 25% Glycerol, 0.1M EGTA, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Purification notes 

Purity: >95% as determined by densitometry.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

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