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Overview

  • Product nameFGFR4 protein (Fc Chimera)See all FGFR4 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 22-201 of Human FGFR4 fused to the Fc region of human IgG1 (aa 93-330) expressed in modified human 293 cells. Swiss ID = P22455.
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin
  • Concentration information loading...
  • Sequence notesTheoretical Sequence: LEASEEVELEPCLAPSLEQQEQELTVALG QPVRLCCGRAERGGHWYKEGSRLAPAGRVR GWRGRLEI ASFLPEDAGRYLCLARGSMIVLQNLTLITGDSLTSSNDDE DPKSHRDLSNRHS YPQQAPYWTHPQRMEKKLHAVPAGN TVKFRCPAAGNPTPTIRWLKDGQAFHGENRIGGIR IPK VDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDT LMISRTPEVTCVVVDVSHE DPEVKFNWYVDGVEVHNAK TKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCRVSNKAL PAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLT CLVKGFYPSDIAVEWESNGQPEN NYKTTPPVLDSDGSF FLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLS PGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83999 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: ab83999 migrates as a broad band between 55 and 65 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified protein which has a predicted molecular mass of 47.1 kDa.

    pI: ab83999 separates into a number of isoforms with a pI between 5.9 and 7.6 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified protein which has a predicted pI of 8.19.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CD 334CD334CD334 antigen
        fc13h 10fc13h10Fgfr 4FGFR-4Fgfr4FGFR4_HUMANFibroblast growth factor receptor 4Hydroxyaryl protein kinaseJTK 2JTK2MGC20292Protein tyrosine kinaseTKFTyrosine kinase related to fibroblast growth factor receptorTyrosylprotein kinase
      see all
  • FunctionReceptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.
  • Tissue specificityExpressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Glycosylated.
    Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization.
  • Cellular localizationMembrane. Isoform 2 may be secreted.

    • Target information above from: UniProt accession P22455 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    FGFR4 protein (Fc Chimera) images

    • Functional Studies - FGFR4 protein (Fc Chimera) (ab83999)
      Functional Studies - FGFR4 protein (Fc Chimera) (ab83999)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangle indicates the theoretical MW and pI of the protein.

    References for FGFR4 protein (Fc Chimera) (ab83999)

    ab83999 has not yet been referenced specifically in any publications.

    Publishing research using ab83999 ? Please let us know so that we can cite the reference in this datasheet

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