You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

FGR 293T Transfected Lysate - (positive control) (ab94154)

Submit a Question

Overview

  • Product nameFGR 293T Transfected Lysate - (positive control)
  • General notesab94154 is a 293T cell transfected lysate in which Human FGR has been transiently over-expressed using a pCMV-FGR plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFgr is a member of the Src family of non-receptor tyrosine kinases. It is expressed in neutrophils, monocytes, macrophages and natural killer cells. Expression of Fgr is developmentally regulated and required in normal myelomonocytic differentiation in vivo. HL-60 cells induced to differentiate with retinoic acid express high levels of Fgr.

    • Applications

    Our Abpromise guarantee covers the use of ab94154 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • FGR 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - FGR 293T Transfected Lysate - (positive control) (ab94154)
      SDS-PAGE - FGR 293T Transfected Lysate - (positive control) (ab94154)
      ab94154 at 15µg/lane on an SDS-PAGE gel.
    • Western blot - FGR 293T Transfected Lysate - (positive control) (ab94154)
      Western blot - FGR 293T Transfected Lysate - (positive control) (ab94154)
      All lanes : Anti-FGR antibody (ab58228) at 1/500 dilution

      Lane 1 : FGR 293T Transfected Lysate - (positive control) (ab94154)
      Lane 2 : 293T Non Transfected Lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for FGR 293T Transfected Lysate - (positive control) (ab94154)

    ab94154 has not yet been referenced specifically in any publications.

    Publishing research using ab94154 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab94154

    There are currently no Questions for ab94154.
    Please use the link above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"