FGR 293T Transfected Lysate - (positive control) (ab94154)

Overview

  • Product nameFGR 293T Transfected Lysate - (positive control)
  • General notesab94154 is a 293T cell transfected lysate in which Human FGR has been transiently over-expressed using a pCMV-FGR plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWBmore details

Properties

  • BackgroundFgr is a member of the Src family of non-receptor tyrosine kinases. It is expressed in neutrophils, monocytes, macrophages and natural killer cells. Expression of Fgr is developmentally regulated and required in normal myelomonocytic differentiation in vivo. HL-60 cells induced to differentiate with retinoic acid express high levels of Fgr.

Applications

Our Abpromise guarantee covers the use of ab94154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • FGR 293T Transfected Lysate - (positive control) images

    • ab94154 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-FGR antibody (ab58228) at 1/500 dilution

      Lane 1 : FGR 293T Transfected Lysate - (positive control) (ab94154)
      Lane 2 : 293T Non Transfected Lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for FGR 293T Transfected Lysate - (positive control) (ab94154)

    ab94154 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"