Overview

  • Product nameAnti-FGR antibody
    See all FGR primary antibodies
  • Description
    Mouse monoclonal to FGR
  • Tested applicationsWB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MGCVFCKKLE PVATAKEDAG LEGDFRSYGA ADHYGPDPTK ARPASSFAHI PNYSNFSSQA INPGFLDSGT IRGVSGIGVT LFIALYDYEA , corresponding to amino acids 1-90 of Human FGR

Properties

Applications

Our Abpromise guarantee covers the use of ab58228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNon-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1.
    • Tissue specificityDetected in neutrophils, monocytes and natural killer cells (at protein level). Detected in monocytes and large lymphocytes.
    • Involvement in diseaseMutations that cause aberrant kinase activation can confer oncogene activity and promote aberrant cell proliferation.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • Post-translational
      modifications
      Ubiquitinated. Becomes ubiquitinated in response to ITGB2 signaling; this does not lead to degradation.
      Phosphorylated. Autophosphorylated on tyrosine residues. Becomes phosphorylated in response to FCGR2A and/or FCGR2B engagement, cell adhesion and signaling by ITGB2. Prior phosphorylation at Tyr-523 by SRC inhibits ulterior autophosphorylation at Tyr-412.
    • Cellular localizationCell membrane. Cell membrane. Cell projection > ruffle membrane. Cytoplasm > cytosol. Cytoplasm > cytoskeleton. Mitochondrion inner membrane. Mitochondrion intermembrane space. Detected in mitochondrial intermembrane space and at inner membranes (By similarity). Colocalizes with actin fibers at membrane ruffles. Detected at plasma membrane lipid rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • c fgr antibody
      • c fgr protooncogene antibody
      • c src 2 proto oncogene antibody
      • c src2 antibody
      • Fgr antibody
      • FGR_HUMAN antibody
      • FLJ43153 antibody
      • Gardner Rasheed feline sarcoma viral (v fgr) antibody
      • Gardner Rasheed feline sarcoma viral (v fgr) oncogene homolog antibody
      • Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog antibody
      • MGC75096 antibody
      • p55 c fgr protein antibody
      • P55 FGR antibody
      • P55-FGR antibody
      • p55c fgr antibody
      • p58-Fgr antibody
      • p58c fgr antibody
      • p58c-Fgr antibody
      • Proto oncogene c Fgr antibody
      • Proto oncogene tyrosine protein kinase FGR antibody
      • Proto-oncogene c-Fgr antibody
      • SRC 2 antibody
      • SRC2 antibody
      • Tyrosine protein kinase Fgr antibody
      • Tyrosine-protein kinase Fgr antibody
      see all

    Anti-FGR antibody images

    • FGR antibody (ab58228) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymph node.


    • Predicted band size : 59 kDa
      FGR antibody (ab58228) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-FGR antibody (ab58228)

    ab58228 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"